Macromolecule Identification Lab

Question 1

Iodine

Answer 1

tests for: starch
negative: brown
positive: dark purple

Question 2

Biuret

Answer 2

tests for: proteins
negative: royal blue
positive: violet/purple

Question 3

Benedicts

Answer 3

tests for: reducing sugars (all monosaccharides but only some disaccharides; sucrose is negative with Benedicts)
negative: aqua blue
positive: green, orange, or red (the more towards red, the greater the sugar concentration)

Question 4

Sudan IV

Answer 4

tests for: Fats
negative: no red fat globules
positive: red boundary around fat globules