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BIO 2 > M1: GENETIC ENGINEERING > Flashcards

M1: GENETIC ENGINEERING Flashcards

1
Q

classical breeding or genetic engineering

 Involves a direct change to an organism’s genome

A

GENETIC ENGINEERING

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2
Q

classical breeding or genetic engineering

 Works primarily through insertion of genetic material

A

GENETIC ENGINEERING

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3
Q

classical breeding or genetic engineering

 The insertion process in genetic engineering does not occur in nature
a. Laboratory procedures

A

GENETIC ENGINEERING

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4
Q

classical breeding or genetic engineering

 Emphasizes desirable characteristics/gene. These characteristics are not new for the species.

A

CLASSICAL BREEDING

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5
Q

classical breeding or genetic engineering

 Employs processes that occur in nature, such as sexual and asexual reproduction
a. Controlled pollination
b. Mating of animals with desired traits

A

CLASSICAL BREEDING

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6
Q

classical breeding or genetic engineering

 Seeks to achieve expression of genetic material which is already present within a species

A

CLASSICAL BREEDING

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7
Q

classical breeding or genetic engineering

 Develops new varieties of organisms by using the process of selection

A

CLASSICAL BREEDING

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8
Q
  • Deals with the modification of genetic material of an organism
A

GENETIC ENGINEERING

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9
Q
  • Also known as Recombinant DNA Technology
A

GENETIC ENGINEERING

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10
Q

It may be involved in…
1. Introduction of new traits
2. Enhancement of present trait
 Increasing its expression
 Decreasing the inhibitors of the expression

A

GENETIC ENGINEERING

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11
Q
  • Is a technology that is used to insert a human gene into the genetic material of a common bacteria
A

RECOMBINANT DNA

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12
Q
  • To produce proteins encoded by the human gene
A

RECOMBINANT DNA

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13
Q

STEPS OF MOLECULAR CLONING/RECOMBINANT DNA

  1. Identifying which chromosome contain the gene of ___________
  2. Selection of appropriate ______ or vehicle which would propagate the recombinant DNA
  3. Cutting a ________ DNA by ________ enzymes
  4. ________ of the gene of interest with the vector
  5. Transfer of recombinant _________ into the host
  6. Selection process to ______ which cell contain the gene of interest
A

STEPS OF MOLECULAR CLONING/RECOMBINANT DNA

  1. Identifying which chromosome contain the gene of interest
  2. Selection of appropriate vector or vehicle which would propagate the recombinant DNA
  3. Cutting a cleavage DNA by Restriction enzymes
  4. Ligation of the gene of interest with the vector
  5. Transfer of recombinant plasmid into the host
  6. Selection process to screen which cell contain the gene of interest
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14
Q

are those DNA molecules that can carry a foreign DNA fragment when inserted into it

A

vectors

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15
Q

are circular deoxyribonucleic acid (DNA) molecules that replicate independently of the bacterial chromosome

A

plasmids

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16
Q

QUALIFICATION OF A GOOD VECTOR

  1. _________ and easy to ___________
  2. can be ________ into a cell
  3. not _______ to host cell
A

QUALIFICATION OF A GOOD VECTOR

  1. small and easy to isolate
  2. can be introduced into a cell
  3. not toxic to host cell
17
Q

QUALIFICATION OF A GOOD VECTOR

  1. have one or more _____ of _______
  2. have one or more ______ ________ sites
  3. have a ______ _________ (antibiotic resistance gene) which allows recognition of transformants
A

QUALIFICATION OF A GOOD VECTOR

  1. have one or more origins of replication
  2. have one or more unique restriction sites
  3. have a selectable marker (antibiotic resistance gene) which allows recognition of transformants
18
Q

it is an enzyme that causes the joining of two molecules which can form a new chemical bond

19
Q

METHODS TO INTRODUCE DNA INTO CELLS

  1. trans_____________
  2. trans______
  3. ___________ /____ gun
A

METHODS TO INTRODUCE DNA INTO CELLS

  1. transformation
  2. transfection
  3. biolistics/gene gun
20
Q

METHODS OF TRANSFECTION

  1. ________ transfection
  2. _______________
A

METHODS OF TRANSFECTION

  1. chemical transfection
  2. electroporation
21
Q

method to introduce dna into eukaryotic cell

A

transfection

22
Q

method to introduce dna into prokaryotic cell

A

transformation

23
Q

insert gene into the reporter gene

A

insertional inactivation

24
Q

dalawang oprganism na ginagalaw sa genetic engineering

A

DNA AND RNA

25
Q

makes the dna full again

A

origins of replication

26
Q

heart of the plasmid that we can easily cut without harming the plasmid

A

restriction sites

27
Q

where we can get the gene of interest

A

chromosome 11

28
Q

the insulin gene is also called as __p__._

29
Q

most common vector is bacteria specifically _______

30
Q

means closing or sewing ti together

31
Q

temperature for hot bath during chamical transformation

32
Q

how long isb the cold bath in chemical transformation

A

30 minutes

BIO 2 (9 decks)

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