Ligase

Question 1

What ligase testing principle is described based on the extraction of free fatty acids released by lipolysis from the reaction mixture followed by conversion of acid to the cupric salt and we extraction into inorganic phase containing DTC

Answer 1

Spectrophotometery

Question 2

What ligase testing principles described lipase is measured by complex axillary and indicator enzyme system the triarylimidazole Lueko dye is oxidized by hydrogen peroxide in the presence of peroxidase to produce a color dye the substrate used in the ass a permit to detection of carboxylesterase and intestinal lipase activities in addition to pancreatic lipase

Answer 2

Dry slide technique

Question 3

What enzyme functions to break down fats by spreading their molecular chain creating free fatty acid and glycerides

Answer 3

Lipase

Question 4

True or false lipase is affected by this functions of the pancreas and salivary gland

Answer 4

False not the salivary glands

Question 5

Explain the lipase Levells after an acute pancreatitis attack

Answer 5

Lipase will increase within 4-8 hours at 24 hours and decrease within 8 to 14 days

Question 6

True or false lipase levels remain elevated longer than amalyse

Answer 6

True

Question 7

Lipase in conjunction with what other enzyme is used to diagnose acute pancreatitis

Answer 7

Amalyse

Question 8

Why Should hemolysis be avoided when performing lipase analysis

Answer 8

It can cause a false decrease

Question 9

What specimen type can lipase testing be conducted on?

Answer 9

Serum plasma ascetic fluids and pleural fluid