Enzymes

Question 1

Catalyst

Answer 1

A substance that enhances chemical reactions but is not altered by the reaction

Question 2

Substances that are structurally similar to the substrate and will compete with the substrate for the enzye

Answer 2

Competitive inhibitor’s

Question 3

Proteins that act as catalyst to accelerate chemical reactions within cells

Answer 3

Enzyme

Question 4

A substance that An enzyme acts upon

Answer 4

Substrate

Question 5

Substances that will interfere with the activity of the enzyme

Answer 5

Inhibitors

Question 6

Enzymes that can perform catalytic activity without assistance from another substrate

Answer 6

Simple enzymes

Question 7

Enzyme poisons or in activator such as heavy metals or halogen

Answer 7

Non-competitive inhibitor’s

Question 8

Enzymes that require the presence Of a non-protein component to function

Answer 8

Conjugated enzymes

Question 9

Non-protein components that conjugate with enzymes to carry out its function usually a metal

Answer 9

Cofactor

Question 10

True or false all enzymes are found inside sales

Answer 10

True

Question 11

True or false all enzymes are proteins and are affected by the same things that affect any other proteins

Answer 11

True

Question 12

True or false each enzyme has multiple substrate it up acts upon

Answer 12

False Bitch

Question 13

Transfer of electrons from one molecule to another

Answer 13

Oxidoreductases

Question 14

Transfer is functional groups from one molecule to another

Answer 14

Transferases

Question 15

Hydrolyzes a chemical bond

Answer 15

Hydrolases & lysases

Question 16

Catalyzes the structural formation of isomers

Answer 16

Isomerases

Question 17

Join together sugar phosphate backbone of DNA fragments

Answer 17

Ligases

Question 18

List of five variables that can affect enzymatic reaction

Answer 18

Timing temperature PH inhibitors and concentration of reaction

Question 19

An increase of _____ C what affect the enzyme reaction rate

Answer 19

10 degrees

Question 20

True or false enzymes are measured in usual concentration units

Answer 20

False

Question 21

Increase in______ Enzyme levels indicate cellular death and leakage of the enzyme from the sale

Answer 21

LDH

Question 22

What LBH fraction is increased in myocardial infarction and in some hemolytic anemia

Answer 22

LDH 1 & LDH2

Question 23

What do enzymes are considered to be the major cardiac enzymes

Answer 23

LDH &CK

Question 24

What enzyme is a cellular enzyme with a physiological role associated with the adenosine triphosphate generation for contractile or transport systems and is responsible for the production creatine phosphate?

Answer 24

Creatine Kinase

Question 25

What CKI so enzyme is present in heart muscles and in the diaphragm and esophagus

Answer 25

CKMB

Question 26

What enzyme functions to break down complex carbohydrate molecules such as starches by splitting the chemical bonds in the molecular chain

Answer 26

Analyses

Question 27

Functions to remove the ternal phosphate group from inorganic phosphate Ester and an alkaline solution is found in lower concentrations in liver and placental tissue

Answer 27

ALP

Question 28

Belongs to a group of eight times known as Aminotransferase and found I am practically every tissue in the body it is in particularly high concentration in liver and cardiac tissues intermediate in skeletal muscle and the kidney and low in most other tissues

Answer 28

AST

Question 29

Catalyzes the transfer of an amino group between L alamine and L glutamate High concentrations occur in the liver in relatively low concentrations are found in the heart muscle and kidney

Answer 29

ALT

Question 30

Glutathione is the common substrate of this enzyme in the body present in high concentrations and liver kidney pancreas and prostate

Answer 30

GGT

Question 31

Assay in which once the substrate and enzyme are mixed they are allowed to react for a set period of time the reaction is stopped and one absorbance reading is taken

Answer 31

Fixed time assay

Question 32

Mid portion of reaction we are the most accurate measurements are taken

Answer 32

Linear phase

Question 33

Final stage of enzymatic reaction where the enzyme have utilize all of the substrate unsuitable for taking measurements

Answer 33

Substrate depletion\exhaustion

Question 34

Assays begin with a known amount of substrate and measure the amount of substrate that remains after a given period of time

Answer 34

Substrate used up assay

Question 35

Assay in which the progress or rate of the reaction is measured over a period of time multiple absorbance readings are taken at preset timed intervals

Answer 35

Kinetic assay

Question 36

Assays are designed to allow enzymes in the patient specimen to react with a set amount of substrate and quantitate the amount of product formed

Answer 36

Product formed assay

Question 37

The initial period of a reaction where the substrate buffer and cofactors factors are mixed kinetic equilibrium is established

Answer 37

Lag phase

Question 38

Monosaccharides or dissect liberated by amylases reaction with the substrate are measured. Byproducts of amylase action are reducing the substances the enzyme action is directly proportional to the amount of reducing substance produced

Answer 38

Saccharogenic method

Question 39

A dye is coupled to a complex and soluble polysaccharide. The sample is incubated and after a fixed time the color liberated from the dye is split from the original complex and the amount measured the enzyme accent is directly proportional to the amount of color produced

Answer 39

Chromolytic Method

Question 40

A different approach to a chromolytic technique depends on the release of a small water soluble fragment in such a way that the color can be measured continuously reaction is monitored by measurement of liberated chromogen

Answer 40

Continuous monitoring

Question 41

Amos is allowed to act on it starch substrate to which iodine has been attached. Hydrolysis of the starch molecule occurs and iodine is released resulting in a decrease of the initial dark blue color intensity. The greater the Amaylase effectivity the lighter the color of the final solution

Answer 41

Amyloclastic method