Lesson 9: Pseudomonas aeruginosa and Burkholderia species

Question 1

• medium-sized, gram-negative rods (0.5 to 1.0 x 1 to 5um)
• obligate aerobes
• most isolates are oxidase-positive and catalase-positive

Answer 1

Pseudomonas aeruginosa and Burkholderia species

Question 2

are motile by polar flagella

Answer 2

Pseudomonas species and Burkholderia pseudomallei

Question 3

diffusible pigments are produced by?

Answer 3

P. aeruginosa

Question 4

causes glanders

Answer 4

Burkholderia mallei

Question 5

cause melioidosis

Answer 5

Burkholderia pseudomallei

Question 6

causes opportunistic infections

Answer 6

Pseudomonas aeruginosa

Question 7

pigments produced by pseudomonas aeruginosa;

Answer 7

Pyocyanin (blue green)
Pyoverdin (greenish yellow)
Pyorubin (red)
Pyomelanin (brownish black)

Question 8

blue green

Answer 8

pyocyanin

Question 9

greenish yellow

Answer 9

pyoverdin

Question 10

red

Answer 10

pyorubin

Question 11

brownish black

Answer 11

pyomelanin

Question 12

large and flat with serrated edges

Answer 12

P. aeruginosa

Question 13

white and smooth, becoming granular and brown with age

Answer 13

Burkholderia mallei

Question 14

Ranges from smooth and mucoid to rough and dull, becoming yellowish brown with agee

Answer 14

Burkholderia pseudomallei

Question 15

grape like colony odour

Answer 15

P. aeruginosa

Question 16

musty colony odour

Answer 16

B. pseudomallei

Question 17

Infections of P. aeruginosa; Host Disease condition in Cattle

Answer 17

mastitis
metritis
pneumonia
dermatitis
enteritis

Question 18

Infections with P. aeruginosa; Host Disease (condition) in Sheep

Answer 18

Mastitis
Fleece rot
pneumonia
otitis media

Question 19

Infections with P. aeruginosa; Host disease (condition) in Pigs

Answer 19

Respiratory infections
otitis

Question 20

Infections of P. aeruginosa; Host disease (condition) in Horses

Answer 20

Genital tract infections
pneumonia
ulcerative keratitis

Question 21

Infections with P. aeruginosa; Host disease (condition) in Dogs and cats

Answer 21

otitis externa
cystitis
pneumonia
ulcerative keratitis

Question 22

Infection with P. aeruginosa; Host disease (condition) in Mink

Answer 22

Haemorrhagic pneumonia
septicaemia

Question 23

Infections with P. aeruginosa; Host disease (condition) in Chinchillas

Answer 23

pneumonia
septicaemia reptiles (captive)
necrotic stomatitis

Question 24

has both Type II and Type III bacterial secretion system which are used by the bacteria to secrete their virulence factors to invade host cells

Answer 24

Pseudomonas aeruginosa

Question 25

transport irons across cell membranes

Answer 25

siderophores

Question 26

causative agent of glanders

Answer 26

B. mallei

Question 27

is a contagious disease of Equidae characterized by the formation of nodules and ulcers in the respiratory tract or the skin

Answer 27

Glanders

Question 28

is capable of intracellular survival and spread and utilizes actin based motility similar to Listeria monocytogenes

Answer 28

Burkholderia mallei

Question 29

caused by B. pseudomallei

Answer 29

Melioidosis

Question 30

is endemic i tropical and sub tropical regions of south-eastern asia and australia where the organism is widely distributed in soil and water

Answer 30

Melioidosis

Question 31

the aetiological agent shares many putative virulence factors with B. mallei including?

Answer 31

capsule, and type III and Type IV secretion systems