Lesson 11: Pasteurella species, Mannheimia haemolytica and Bibersteinia trehalosi

Question 1

• Small gram-negative rods (0.2 x 1-2um)
• optimal growth
• non-motile, oxidase-positive, facultative anaerobes
• most species are catalase positive

Answer 1

Pasteurella species, Mannheimia haemolytica and Bibersteinia trehalosi

Question 2

is prominent in smears from lesions using the giemsa method

Answer 2

Bipolar staining

Question 3

• commensals in the upper respiratory tract
• respiratory pathogens

Answer 3

Pasteurella species, Mannheimia haemolytica and Bibersteinia trehalosi

Question 4

colonies are round, greyish, shiny and non-haemolytic

Answer 4

P. multocida

Question 5

Colonies of some pathogenic strains are mucoid due to the production of thick hyaluronic acid capsules

Answer 5

P. multocida

Question 6

The colonies have a subtle but characteristics sweetish odour

Answer 6

P. multocida

Question 7

colonies are haemolytic and odourless

Answer 7

M. haemolytica, M. granulomatis, and B. trehalosi

Question 8

colonies are round, greyish, and non-haemolytic

Answer 8

Pasteurella species

Question 9

which are haemolytic

Answer 9

P. testudinis

Question 10

on MacConkey agar, these organism grow as pin-point red colonies.

Answer 10

M. haemolytica and B. trehalosi

Question 11

do not grow on macConkey agar

Answer 11

pasteurella species

Question 12

commercially available biochemical test strips such as?

Answer 12

API 2ONE

Question 13

a yellow slant and yellow butt without H2S production are typical

Answer 13

TSI agar slopes

Question 14

particularly in type A strains, has a major antiphagocytic role and may also function in adhesion in some strains

Answer 14

Capsule

Question 15

The capsule of types A and B, D, F is composed of?

Answer 15

hyaluronic acid
heparin
chondroitin

Question 16

The major virulence factor of P. multocida of type D, which produce atrophic rhinitis in pigs

Answer 16

PMT toxin

Question 17

a cytotoxic protein that stimulates cytoskeletal rearrangements and growth of fibroblasts

Answer 17

PMT toxin

Question 18

Four main virulence factors have beem identified in strains M. haemolytica and B. trehalosi

Answer 18

• adhesins
• capsule
• endotoxin
• leukotoxin

Question 19

which may enhance colonization

Answer 19

adhesins

Question 20

inhibits complement mediated destruction of the organism in serum

Answer 20

capsule

Question 21

alter bovine leukocyte functions and is directly toxic for bovine endothelial cells

Answer 21

endotoxin

Question 22

a pore-forming cytolysin that affects leukocyte and platelet functions when present at low concentrations and causes cytolysis at high concentrations

Answer 22

leukotoxin

Question 23

causative agent of bovine pneumonic pasteurellosis (shipping fever) cattle

Answer 23

M. haemolytica

Question 24

predisposing factors such as overwork, poor body condition and monsoon rains are important in its development

Answer 24

Hemorrhagic septicaemia

Question 25

causative agent of Haemorrhagic septicaemia

Answer 25

Pasteurella multocida serotype B:2

Question 26

characterized by severe bronchopneumonia and pleurisy, occurs mostly commonly in young cattle within weeks of being subjected to severe stress such as transportation, assembly in feedlots and close confinement

Answer 26

Shipping fever

Question 27

causative agent of Bovine respiratory disease

Answer 27

M. haemolytica and P. multocida

Question 28

outbreaks of ovine pneumonic pasteurellosis are usually caused by M. haemolytica whereas P. multocida tends to produce sporadic cases of the disease

Answer 28

Pasteurellosis in Sheep

Question 29

Factors that predispose to clinical disease are poorly understood and may include adverse climatic conditions or concurrent infections with respiratory viruses such as?

Answer 29

parainfluenzavirus 3

Question 30

toxigenic strains of P. multocida type D or A cause a severe, progressive form of atrophic rhinitis

Answer 30

Atrophic rhinitis of pigs

Question 31

a primary avian pasteurellosis caused by P. multocida capsular types A and F.

Answer 31

Fowl cholera

Question 32

it is highly contagious and affects both domestic and wild birds but especially turkeys

Answer 32

Fowl cholera

Question 33

in rabbits is a common, recurring, purulent rhinitis in rabbits which is caused by type A strains of P. multocida

Answer 33

Snuffles

Question 34

is a commensal in the upper respiratory tract of healthy carrier rabbits

Answer 34

Pasteurella multocida

Question 35

clinical disease is often precipitated by stress factors such as overcrowding, chilling, transportation, concurrent infections and poor ventilation, resulting in high levels of atmospheric ammonia

Answer 35

Snuffles