Lesson 13 - Calculating genetic biodiveristy Flashcards
Why do scientists calculate genetic biodiversity?
- Monitor health of the population and ensure its long-term survival.
Genes vs alleles?
- All members of the same species have have the same genes.
- Have different alleles.
More alleles in a population…
greater genetic biodiversity.
Factors that affect genetic biodiversity:
Increase:
- Interbreeding between different populations. –> alleles transferred between different populations (gene flow).
- Mutations in the DNA of an organism, creating new allele.
Decrease:
- Selective breeding
- captive breeding programmes
- Rare breeds
- Artificial cloning
- natural selection
- genetic bottleneck
- Founder effect
- Genetic drift
How do scientists measure genetic biodiversity?
- Measuring polymorphism. (eg. immunoglobulin gene for determining blood types)
Three alleles for determining blood type?
I^A - resulting in the production of antigen A
I^B - resulting in the production of antigen B
I^O - resulting in the production of neither antigen
If a gene is not polymorphic, it is…
monomorphic –> basic structure of individuals within a species remains constant.
Equation to work out the proportion of genes that are polymorphic
proportion of polymorphic gene loci =
number of polymorphic gene loci / Total number of loci
Technique used to separate fragments of DNA based on size?
Gel electrophoresis
- Restriction enzymes cut DNA into smaller pieces - placed in gel.
- Gel is placed between positive and negative electrodes - causes negatively charged DNA to move towards the positive side.
- Smaller fragments move further through gel.
- Banding pattern produced. –> compare.