LESSON 12: CULTURE MEDIA Flashcards
Laboratory diagnosis of an infection is usually confirm by:
- ISOLATING
- CULUTURING microorganicm by artificial media
Bacteria and fungi are cultured either by:
- LIQUID (broth)
- SOLID (agar)
-pioneered the use of agar as a base for culture media as suggested by Fannie Eilshemius Hesse
ROBERT KOCH
-developed the petri dish.
RICHARD PETRI
-a container for solid culture media.
PETRI DISH
-developed the pour plate method and was the first to use solid culture media
for culture of bacteria.
KOCH
-A nutrient material prepared for the growth of microorganisms in a laboratory is called a?
CULTURE MEDIUM
- When microbes are introduced into a culture medium to initiate growth.
INOCULUM
-The microbes that grow and multiply in or on a culture medium are referred to a?
CULTURE
-is the process of growing microorganisms in culture by taking bacteria from the infection site by some means of specimen collection and growing them in the artificial environment of the laboratory.
CULTIVATION
-a laboratory culture containing a single species of organism.
PURE CULTURE
-is prepared from a variety of seaweeds and is now
universally used for preparing solid media.
AGAR
-agars melt at about ?
95 degree celsius
-agars solidify only when cooled at?
42 degree celsius
-accessory growth factors of peptone:
- riboflavin
- nicotinic acid
-. It is a complex mixture of partially digested prote
PEPTONE
-It contains a wide range of amino acids, growth factors and inorganic salts.
- is used mainly as a comprehensive source of growth
factors and may be substituted for meat extract in culture media
YEAST EXTRACT
-It consists mainly of maltose (about 50%), starch, dextrins and glucose, and contains about 5 percent of proteins and protein breakdown products, and a wide range of mineral salts and growth facto
MALT EXTRACT
-hese are used for enriching culture media. Either human or animal blood can BE USED.
BLOD SERUM
- In this type of media nutrients are dissolved in water, and bacterial growth is indicated by a change
in broth’s appearance from clear to turbid.
LIQUID OR BROTH MEDIA
-are made by adding a solidifying agent to
the nutrient.
SOLID MEDIA or AGAR
-Petri dish containing the agar is referred to as?
AGAR
-are clones of cells originating from a single bacterial cell.
CLONES
-the simplest and most common medium used routinely in diagnonistic laboratories.
NUTRIENT AGAR by adding 2% of agar to nutrienth broth
-for special purposes where agar is added to media in
concentrations that are too low to solidify them
SEMI SOLID MEDIA
-at this percent it yields a
semisolid medium through which motile, but not non motile, bacteria may spre
0.2- 0.5 %
-This media include nutrient broth and
peptone water, which form the basis of other media.
BASAL MEDIA or SIMPLE MEDIA
-is an example of a simple liquid medium that consists
of peptone, meat extract, sodium chloride, and water
NUTRIENT BROTH
-Addition of 0.5% glucose to nutrient broth makes it?
GLUCOSE BROTH
-Is an example of a simple solid medium.
- The medium is
used routinely for isolation of many bacteria from clinical specimens.
NUTRIENT AGAR
-: a media that contain some ingredients of unknown
chemical composition.
COMPLEX MEDIA
-s a synthetic kind of medium which contains known quantities of all ingredients used but does not contain any animal, yeast, or plant tissue
DEFINED MEDIA
-is an enriched medium in which nutritionally rich whole
blood supplements constitute the basic nutrients.
BLOOD AGAR
-is enriched with heat-treated blood (80°C), which turns brown and gives the medium the color for
which it is named.
CHOCOLATE AGAR
-These are solid media that contain substances that inhibit
the growth of all but a few bacteria but at the same time facilitate isolation of certain bacteria.
SELECTIVE MEDIA
-selective for the isolation of Vibrio cholerae
Thiosulfate citrate bile salt sucrose agar
-selective for enteric bacilli, such as Salmonella spp. and Shigella spp..
Deoxychocolate citrate agar (DCA)
-this medium is intended for Myobacterium tuberculosis.
LJ Medium
-this 2 medium is selective for Gram-negative bacteria.
Hektoen enteric agar (H.E)
& Xylose lysine desoxychocolate agar
-this medium is selective for Gram-positive bacteria
Mannitol salt agar
-selective for certain Gram-negative bacteria, such as Legionella pneumophila
Buffered charcoal yeast extract agar
-are liquid media that stimulate the growth of certain bacteria or suppress the growth of others for isolation of desired pathogenic bacteria.
ENRICHMENT MEDIA
- this bacteria are present in feces, tend to overgrow pathogenic ones in stool specim
Escherichia coli.
-distinguish one microorganism from another growing on the same media by their growth characteristics.
INDICATOR MEDIA
-differential for lactose
and sucrose fermentation.
EOSIN METHYLENE BLUE
-differential for lactose fermentatiuon
MacConkey
-differential for mannitol fermentation
Mannitol salt agar
-differential for lac operon mutants for detection of recombinant strains of bacteria for
study in molecular biology.
X-gal plates
-media are used to maintain the viability of
certain delicate organisms in clinical specimens during their transport to the
laboratory.
TRANSPORT MEDIA
-what medium is for Neisseria gonorrhoeae
Stuart’s transport medium
-in microbiology denotes any fermentable substance, such as glucose, sucrose, lactose, and mannitol that is routinely used
SUGAR MEDIA
-sugar media is contains how many % of sugar?
1%
-can be used for the short-term storage of bacterial
cultures.
REFRIGERATION
- a process in which a pure culture of microbes is
placed in a suspending liquid and quick-frozen at temperatures ranging from - 50º to-95°C.
DEEP FREEZING
-a suspension of microbes is quickly frozen at temperatures ranging from -54º to -95ºC, and the water is removed by a high vacuum (sublimation).
LYOPHILIZATION
-The organisms can be
revived at any time by hydration with a suitable liquid nutrient medium
LYOPHILIZATION
- is a common method of preserving strains
COLD STORAGE
-number of drying methods for drying suspensions of bacteria for preservation purposes have been developed.
DRYING METHOD
