LESSON 11: ASEPTIC TECHNIQUE

Question 1

The area should be kept clean
and not contain equipment other than that related to culture. Non sterile activities
should not be carried

Answer 1

QUIET AREA

Question 2

: should be avoided as much as possible to prevent generation of a bridge of
liquid between the outside of the bottle and the inside, which may permit
contamination to enter tHE BOTTLE.

Answer 2

POURING

Question 3

standard glass or disposable plastic pipettes are the easiest way to
manipulate liquids. Mouth pipetting should be avoided to avoid mycoplasmal
contamination and prevent hazard to the operator.

Answer 3

PIPETTING

Question 4

-an equipment that protect work surface from dust and contamination by a
constant, stable flow of filtered air passing over the work surface

Answer 4

SAFETY CABINET

Question 5

-Humidified incubators are a major source of contamination. Incubators should
be regularly cleaned with detergent and then swabbed with 70% alcohol and cleared
of any inappropriate contents. The humidifier tray at the bottom of the incubator can
be supplemented with a fungicide to retard fungal growth

Answer 5

INCUBATORS