LABORATORY LESSON 1 Flashcards
Students in microbiology must understand and follow the rules in the lab. Student should be:
- familiar with the location
- use of the safety equipment in the laboratory
- be aware of all exits from the room.
laboratories classified as:
1.biosafety level I (BSL)
2. biosafetY leve (BSL2)
organisms used in BL2
laboratory are classified as:
CAUSATIVE AGENTS
- is the application of safety precautions that reduce a laboratorian’s risk
of exposure to a potentially infectious microbe and limit contamination of the work
environment and, ultimately, the community.
BIOSAFETY
-organisms are well-characterized strains of microorganisms not known to cause disease in healthy human adults. Precautions in this lab include general laboratory safety.
BSL1
example of organism in bsl 1:
Escherichia coli,
Staphylococcus xylosus,
Bacillus mega
organisms are moderate-risk microorganisms associated with less serious human diseases whose potential for transmission is limited and a proven treatment for the disease exist.
BSL2
organisms are high-risk microorganisms with a true potential for infection by aerosols and in which the resulting disease may have serious or lethal consequences
BSL 3
example of organism in BSL 3:
Mycobacterium tuberculosis
Foot-and-mouth disease virus,
Bacillus
organisms are easily transmitted, very-high risk
microorganisms which cause life-threatening diseases for which there is no vaccine or
therapy.
BSL 4
“space suits” with an external oxygen supply, and precautions such as chemical showers must
be taken before exiting in this bsl:
BSL 4
Example of BSL4
Ebola virus
Marburg virus
Lassa fe
Avian flu virus
Steps of risk management process:
- IDENTIFY HAZARAD AND RISK
- EVALUATE THE RISK
- IMPLEMENT A MITOGATION PLAN AS NEEDED
- EVALUATE EFFECTIVENESS OF CONTROL
starting point for the laboratory investigation is the taking of,
SAMPLES
between samples. The samples should
be:
- carefully packaged
2.labeled - transmitted to the laboratory by the fastest
4.practicable method, with the appropriate temperature cont
Sample collection from live animals:
- BLOOD
- SKIN
- GENITAL TRACT & SEMEN
- EYE
- NASAL DISCHARGE
- MILK
types of sample:
- SAMPLE COLLECTION FOR LIVE ANIMALS
- SAMPLE COLLECTION AT POST MORTEM
- ENVIRONMENTAL & FEEDING SAMPLING
- INFORMATION TO BE SENT W/ SAMPLE
- PRESERVATION OF SAMPLE FOR PROLONGED STORAGE
to examine microorganisms which can’t
be seen by naked eye
MICROSCOPE
to heat or boil solution in laboratory
BUNSEN BURNER
2 equipment use to sterilize the equipment medias and other solutions
- AUTOCLAVE
- PRESSURE COOKER
to preserve the samples, media, reagents
and other specimen
REFRIGERATOR
creates a rotating magnetic field
MAGNETIC STRRING PLATE
used for bacterial or fungal cultures
INCUBATORS
to rescue the exposure of the operator
and the lab contamination
SAFETY CABINET
used to inoculate test
samples into culture media for bacterial or fungal
cultures, antibiograms
INOCULATING LOOP
to act as a supporting container to hold the
culture medium.
PETRI DISH
2 types of test tube:
- TEST TUBE
- TEST TUBE RACK
to measure and prepare media
FLASK
to measure and transfer the solution
BEAKER
to measure the weight of an
specimen
WEIGHING SCALE
to transfer quantified volume of specimen
or solution.
PIPETTOR
pipette tips are used as:
Almost all the pipettes require pipette tips for performing their intended work
Used to heat medium gently (to around
45-55℃) during media preparation
WATER BATH
partner of GLASS SLIDES?
COVER SLIPS
this bottles protect chemicals sensitive to light and ultraviolet rays.
AMBER BOTTLES
for storage of media, regeants and other
materials
CUPBOARDS
Storage cabinet for personal protective equipment
PPE’s
