LESSON 10: CULTURE METHODS Flashcards
-is the population of bacteria grown in the laboratory.
CULTURE
-contains one single type of bacteria.
PURE CULTURE
-contain two or more different bacteria.
MIXED CULTURE
-where bacterial cultures must be periodically transferred to a new media to keep bacterial population growing.
SUBCULTURE
-means using practices and procedures to
prevent contamination from pathogens.
ASEPTIC TECHNIQUE
-the most effective way to isolate a single type of
bacteria from a source that contains many by diluting the individual cells by spreading them over the surface of an agar plate using a platinum or inoculating loop of 2-4 mm diameter.
STREAK PLATE METHOD
-also called as CARPET CULTURE
LAWN CULTURE
lawn culture is used for 3 reasons:
- ANTIBIOTIC SUSPECTIBILITY TESTING by diffusion method
- BACTERIOPHAGE TYPING
- preparations of BACTERIAL ANTIGENS & VACCINES
-Stroke culture provides a PURE GROWTH of bacteria for carrying out slide agglutination and other diagnostic test, it’s carried in TUBES containing slanted nutrient agar slopes.
STAB CULTURE
- is used to determine approximate
number of viable organisms in liquids, suchs as water or urine.
POUR PLATE CULTURE
-a deep culture of agar or gelatin through which the inoculum is evenly distributed by shaking before the medium is solidified and which is used chiefly for the demonstration of anaerobic colonies.
SHAKE CULTURE
-this culture not provide a pure culture from mixed inocula—the major disadvantage, nor identify a bacteria
LIQUID CULTURE
-liquid culure can be used for:
- BLOOD CULTURE
- DILTUION IN THE MEDIUM
- LARGE YIELDS CULTURE
in aerobic culture this organism requires CO2 w/ 5-10% in in the air in which they are grown.
1.Brucella abortus
2.Capnophilic streptococci
3. Pneumococcus
4. Gonococcus
Aerobic culture done in an incubator under normal atmospheric condition, what is the standar practice temperature?
37 degree celsius
-a method routinely employed in clinical bacteriology and enables the isolation of distinct colonies which may be picked out, if necessary for further
purification and study
SURFACE PLATING
-selective media for diphteria bacillus
tellurite media
-favor the multiplication of particular species as a step towards their isolation in
pure culture.
ENRICHMENT MEDIA
-enrivchment media for Salmonella sp.
SELECTIVE BROTH
-contain ingredients that change in appearance with particular organisms and so assist their isolaion.
INDICATOR MEDIA
-indicator media for Clostridium sp.
WILLIS & HOBBS MEDIUM
-can be used to separate spores from vegetative bacilli but does not guarantee that spores will germinate under subsequent cultural conditions.
HEATING WITH HIGHER TEMPERTURE FOR SHORTER PERIODS.
This method is the standard
practice for the isolation of tubercle bacilli from sputum and other clinical specimens, by treatment with alkali, acid or other substances to which most commensals are
susceptible but tubercle bacilli are resistant.
PRETREATMENT OF SPECIMENS W/ APPROPRIATE BACTERICIDAL SUBSTANCE
-Only thermophilic bacteria at:
60ºC.
-of a tube of semisolid agar, with a narrow tube open at both ends placed in the center of the medium in such a way that it projects above the
level of the agar.
CRAIGES TUBE
-also serves the same purpose, inoculation being performed in one
limb and the subculture taken from the other. This method can also be used to obtain
phase variants in Salmonella species
U-TUBE
-Is inoculated subcutaneously into a mouse, the animal dies of pneumococcal septicemia in 12 to 48 hours and the organism can be obtained in
pure culture from the heart blo
PNUEMOCOCCUS
-can be distinguished from other aerobic
sporulating bacilli by inoculation into mice or guinea pigs. Anthrax bacilli produce a
fatal septicemia and may be cultured pure from the heart blood
ANTHRAX BACILLI
-s can be isolated from
contaminating organisms by inoculation of an infected specimen into a guinea pig. The tubercle bacillus is found in a pure state in the resulting lesion
TUBERCLE BACILLUS
-Bacteria of differing sizes may be separated by the use of selective this,
-this are widely used for separating viruses from bacteria
FILTERS
