Lecture Nov 4 (8.3, 9.1)

Question 1

What are the 4 diff types of point mutations

Answer 1

Silent, Missense, Nonsense
Frameshift

Question 2

Which are the 2 most impactful mutations?

Answer 2

Frameshift and nonsense (making a stop codon)

Question 3

When won’t a frameshift have an impact?

Answer 3

When 3 codons are deleted/inserted

Question 4

When wont a nonsense mutation have an effect on the codon?

Answer 4

When the mutation occurs at the end of the coding strand

Question 5

What part of the tRNA structure does the amino acid get connected to?

Answer 5

the A of the ACC end of the tRNA

Question 6

What part of the tRNA does

Question 7

Can an Inosine (??) get base pair to Adenine?

Answer 7

Yes, only when tRNA interaction 3 (mRNA) and 1 (tRNA).
Wobble position.

Question 8

Messenger RNA purpose

Question 9

Ribosomal RNA purpose

Question 10

Transfer RNA purpose

Question 11

What is the seqe3unce at the 3’ end of the tRNA?

Answer 11

ACC

Question 12

3’ CCA sequenc

Question 13

What 2 sites does amino acyl tRNA have?

Answer 13

editing site and synthesizing

Question 14

What is the synthesis site?

Answer 14

Amino acids which are too large compared to the correct one can not enter. The correct amino acid (or anything smaller) enters and gets attached to tRNA.

Question 15

What is the editing site?

Answer 15

After exiting the synthesis site, incorrectly charged amino acids enters the editing site and gets cleaved off. Correct amino acid can not enter the editing site

Question 16

How do you name a tRNA which is supposed to carry Met

Answer 16

tRNA^Met

Question 17

How do you name a tRNA that is carrying Met

Answer 17

Met-tRNA^Met