For exam

Question 1

in agarose gel dsDNA is seperated based on

Answer 1

number of base pairs, so size (ONLY)

Question 2

When does DNA Polymerase 1 come in? What does it do

Answer 2

DNA Polymerase 1 comes AFTER DNA Replication is complete.
removes RNA primers and replaces with DNA.

Question 3

After DNA Replication is done, what enzyme joins the phosphodiester bonds?
(fill in gaps)

Answer 3

DNA ligase

Question 4

Heat shock occurs at what temperature?

Answer 4

42

Question 5

39. Which of these proteins are not found in eukaryotic chromatin?

The options are: a) Histone A1 b) eIF4G c) General TFIID d) RNA polymerase II e) DNA ligase

Answer 5

Answer: b) eIF4G
because this is used in translation

Question 6

How is proofreading done

Answer 6

DNA polymerase III will use its 3’ to 5’ exonuclease activity to delete the incorrect base
* ε-subunit

Question 7

Alpha sheet amino acids per turn

Answer 7

3.6 amino acids per turn
Each amino acid= 100 degrees

Question 8

Beta sheet seperation per turn (LENGTH of 1 turn of beta sheet)

Answer 8

0.7