Lecture 6 - Nitrogen Metabolism Part 2 Flashcards
when does amino acid catabolism occur
Excess protein is consumed (high protein diet)
Insufficient dietary protein (Kwashiorkor)
Insufficient dietary energy (starvation)
what happens to carbon backbones and amino groups in amino acid catabolism
carbon skeletons enter the main energy pathways and amino groups are processed to urea in the liver for excretion
what is another word for aminotransferase
Transaminase
what is the co factor that is very important for the function of transaminase enzyme
pyridoxal phosphate
what vitamin is pyridoxal phosphate derived from
vitamin B6
where is pyridoxal phosphate found in the transaminase enzyme
Pyridoxal phosphate is found in the active centre of the transaminase enzyme
what is pyridoxamine phosphate
when the amino group from the amino acid has been transferred to the pyridoxal phosphate
once the amino group has been transferred and pyridoxamine phosphate has been formed, what will then occur
that amino acid will be transferred to another keto acid and will form new a-amino acid based off what the keto acid was
what part of the body has the highest concentrations of aminotransferases
the liver
what is the corresponding keto acid to aspartate
oxaloacetate
what is the corresponding keto acid to alanine
pyruvate
what is the corresponding keto acid to glutamate
a-ketoglutarate
what is a characteristic of aminotransferase reactions and what do they depend on
Reactions are reversible and dependent on concentration of substrates
aminotransferases are specific for what and what does that mean (use alanine as an example)
aminotransferases are specific for the donor amino acid,
example: alanine aminotransferase (ALT) primarily catalyzes the transfer of an amino group from alanine to a keto acid
what do most aminotransferases only accept as their donor keto acid
αketoglutarate or oxaloacetate