Lecture 6 - Antibody Effector Function Flashcards
Direct and indirect antibody effector functions
Direct - Function of Fab, neutralisation
Indirect - Involves Fc, targets bound antigen for phagocytosis, etc
Example of antibody direct mechanism
Antibody binds to influenza haemagglutinin
Influenza is now unable to enter host cell
Example of antibody indirect mechanism
Antibody binds to target antigen
If IgE, stimulates mast cell degranulation
Opsonisation
1)
2)
3)
1) Antibody binds to taret antigen
2) Bind to phagocyte by FcR
3) Phagocytosis
What does ADCC stand for?
Antibody-dependent cell-mediated cytotoxicity
Which receptor on a natural killer cell binds to antibody opsonising antigen?
FcgRIII (CD16)
Another name for FcgRIII
CD16
What is CD16?
IgG receptor on the surface of NK cells
What triggers NK cell killing?
Crosslinking of CD16/FcgRIII receptors
Mast cell degranulation
1)
2)
3)
1) Mast cell is ‘sensitised’ - IgE coats mast cell, binds to FceR
2) IgE Fab region comes into contact with allergen
3) Crosslinking of FceR leads to degranulation
Does a mast cell have a monoclonal or polyclonal antibody coating?
Polyclonal.
Can respond to several different allergens
Probably clumps o fmonoclonal antibodies in different places to allow crosslinking, clumping of antibodies
FcgRI affinity
High
FcgRIIA affinity
Low
FcgRIIB affinity
Low
FcgRIIIA affinity
Low
FcgRIIIB affinity
Low
FcgRI cell distribution
Macrophages, neutrophils
FcgRIIA cell distribution
Macrophages, neutrophils
FcgRI function
Cell activation, phagocytosis
FcgRIIA function
Cell activation, phagocytosis