Lecture 19 - Early T Lymphocyte Maturation Flashcards
What does the absence of a thymus result in?
DiGeorge syndrome, thymic aplasia
What happens to the thymus in puberty?
It involutes
What is the thymus made of in adults?
Mostly fat
Strange feature of mice without a thymus
They are bald. Mechanism unknown
Which type of organ is the thymus?
Parenchymous
How would you go about finding if new T cells are being made?
Search for excision circles of DNA.
Implies that RAG recombination is occurring
What happens to T cell development in puberty?
It drops dramatically, but doesn’t cease.
Some new T cells are made throughout life
How do thymocytes enter the thymus?
Through high endothelial venules
Which cells develop in the bone marrow and make their way to the thymus?
Thymocytes
What initiates T cell fate decision and proliferation in thymocytes?
Interaction with thymic stroma cells
Three principal fates of thymocytes
1) Alpha/beta T cells
2) Gamma/delta T cels
3) Invariant NK T cells
Alpha/beta T cells
Conventional T cells, further differentiate into CD4 or CD8
Gamma/delta T cells 1) 2) 3) 4)
1) Primarily located at epithelial, mucosal sites
2) Lack CD4, CD8
3) Don’t undergo positive or negative selection
4) Produced earlier in development than other T cells
What is T cell maturation tied to?
The histological structure of the thymus
What is the state of the TCR locus in thymocytes when they enter the thymus?
Germline configuration
Unrecombined