Lecture 26 - Complement Proteins II Flashcards
Complement components that act as opsonins
C3b, C4b
Complement receptors involved in phagocytosis
CR1, CR3
Another name for CR1
CD35
Another name for CR3
Mac-1
CD11bCD18
Cells that express CR1 and CR3 1) 2) 3) 4)
1) Macrophages
2) Neutrophils
3) Follicular dendritic cells
4) Erythrocytes
What do CR1 and CR3 have a high affinity for?
C3b, iC3b, C4b
What does CR3 bind?
iC3b
iC3b
Breakdown product of C3b that forms on cell membranes
inactive C3b
What enhances C3b phagocytosis?
Specific IgG also binding to microbe
Role of CR1 on erythrocytes
Binds to C3b/C4b opsonised microbes, transports them to the spleen, where they are destroyed
Erythrocyte is not destroyed in this process.
CR2 1) 2) 3) 4)
1) CD21
2) Complement receptor on B cells
3) Complexes with CD19 and CD81
4) Provides a second signal for B cell activation
Which cells express CR2?
B cells
Follicular dendritic cells
What does CR2 bind?
iC3b, C3dg antigen/antibody complexes
What is C3dg?
A C3 breakdown product
CR4
1)
2)
3)
1) Dimer of CD11c and CD18
2) Present on dendritic cells
3) Similar function to CR3
Role of complement in B cell activation
1)
2)
3)
1) Antigens coated by C3dg bind IgM and CR2
2) Boosts phosphorylation of ITAM residues on Igalpha and Igbeta
3) This amplifies BCR signalling
Which complement components are anaphylatoxins?
C3a, C5a
Effects of C3a and C5a
Anaphylatoxins
Cause systemic inflammation, which in extreme cases resembles anaphylactic shock
Where are C3a and C5a receptors found?
1)
2)
3)
1) Mast cells
2) Endothelial cells
3) Phagocytes
How do C3a and C5a cause systemic inflammation?
1)
2)
3)
1) Bind to mast cells, cause them to release TNFa, histamine
2) Bind to endothelial cells, induce vascular leakage
3) C5a is a chemotactant. Attract neutrophils, monocytes