Lecture 6 Flashcards
1
Q
An A260/A280 of ______ is considered good for DNA purifications
A
~1.8
2
Q
Bradford Assay
A
measure of colour change when Coomassie Blue dye binds to protein; quick, simple, 1mg/mL
3
Q
more protein =
A
more blue
4
Q
Absorbance can vary by…
A
- amino acid composition
- protein structure
5
Q
Biuret Assay
A
measure of colour change when Copper II Sulfate reacts with proteins under alkaline conditions; takes time as Cu reacts with peptide bonds, 10mg/mL
6
Q
solution turns _______ in Biuret Assay
A
purple
7
Q
technological miniaturization
A
- no cuvette spec
- sample of 5microliter to 0.5 microliter
- from 200nm to 900nm in 5s.
- sample can be recovered due to hydrophobic surface
8
Q
First and quickest step in identifying a bacterium
A
looking under a microscope
9
Q
prokaryotes
A
- mostly circular chromosomes
- no nucleus or organelle
- peptidoglycan cell walls
- binary fission
10
Q
eukaryotes
A
- linear chromosomes in nuclear membrane
- organells
- polysaccharide cell walls
- mitosis
11
Q
coccus/cocci
A
spherical
12
Q
bacillus/bacilli
A
rods
13
Q
vibrios, spirillas, spirochetes
A
long and bendy
