Lecture 5 Flashcards
1
Q
spectrophotometry
A
- spectroscopy + photometry
- study of interaction of light on a sample
- can tell us what compounds may be present, and in what quantities
2
Q
spectroscopy
A
study of absorption and emission of radiation (light)
3
Q
photometry
A
the measurement of intensity of radiation
4
Q
light is made of…
A
photons
5
Q
higher energy
A
shorter wavelength (blue)
6
Q
lower energy
A
longer wavelength (red)
7
Q
electrons in molecules exist in…
A
orbits/shells
8
Q
photons of the correct wavelength can …
A
bump up electrons
9
Q
Light energy can…
A
- generate heat
- produce fluorescence
10
Q
cuvette
A
tube that holds the sample
11
Q
transmittance
A
proportion of light that the sample alllows to pass
12
Q
absorbance
A
the amount of light that sample blocks
13
Q
blank
A
a comparison sample with none of the measured substance
14
Q
standard curve
A
a series of known samples used to generate comparison data
15
Q
A (absorabance) =
A
log(1/T)
