Lecture 1 + 2 Flashcards

1
Q

hypothesis

A

a testable statement; falsifiable

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2
Q

controlled variables

A

ideally don’t change, common to all treatments

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3
Q

independent variables

A

what you are altering in the experiment

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4
Q

dependent variable

A

what you are observing in the experiment; respond to changes in independent variable

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5
Q

positive controls

A

an experimental treatment which will give the desired result

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6
Q

negative controls

A

an experimental treatment which will not give desired results

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7
Q

false positive

A
  • shown by negative control

- something wrong with machine/sample

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8
Q

false negative

A

shown by positive control

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9
Q

single blind

A

one knows

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10
Q

double blind

A

both don’t know

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11
Q

causation

A

A caused B

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12
Q

correlation

A

A + B, but there could be a C that affected things

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13
Q

sensitivity

A

min. amount of X needed to record a positive result

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14
Q

specificity

A

a positive result only comes from a truly positive sample

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15
Q

random error

A

new error introduced with each measure (weather, temp)

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16
Q

systematic error

A

error that is consistently present (machine)

17
Q

accuracy

A

how close to true value

18
Q

precision

A

how reliable; comparing to other measurements

19
Q

Realistically, any lab manipulations with have…………sig figs

A

2, maybe 3

20
Q

pH meter

A
  • glass probe permeable to H+

- current by probe compared against standards to report a pH value

21
Q

scale

A
  • to weigh chemicals

- must be tared/zeroed with empty container

22
Q

volumetric pipette

A

measure just one volume

23
Q

graduated pipette

A

lines along length

24
Q

some pipettes are ______, some arent

A

blow out

25
Q

micropipettes

A

volumes of 10 to to 0.0002mL, disposable tips, two stop-plunger

26
Q

1mL = ___ul

A

1000 microliter

27
Q

Dilution equation

A

c1v1=c2v2

28
Q

[H+][OH-]=

A

10^-14

29
Q

pH=

A

-log[H+]

30
Q

pKa

A

equal amounts of the acid and conjugate base

31
Q

A buffer pair is only effective …

A

+/- 1 pH unit from pKa