Lecture 24 Flashcards
1
Q
of copies =
A
(2^n - 2n)x
- n= # of cycles
- x= # of copies to start
2
Q
initial denaturation
A
95 for 1-10 mins
-More G-C pairs = longer time needed
Less template = Longer time
3
Q
denaturation
A
94-95 for 30-120s
4
Q
annealing
A
40-65 (primer specific) for 30-120s
5
Q
extension
A
68-78 (standard 72) for N mins (N=product length in kb)
6
Q
repeat 2,3,4, for
A
30-40 cycles, hold at 4
