lecture 33 - oxidative phosphorylation Flashcards
What are the 2 energetic gradients across the inner mitochondrial membrane as a result of the proton gradient created by the ETC?
A chemical/pH gradient, an electrical gradient
What is the pH/chemical gradient across the inner mitochondrial membrane a result of?
Different H+ concentration on either side of the membrane due to the ETC
What is the electrical gradient across the inner mitochondrial membrane a result of?
A charge difference across the membrane - intermembrane space is positive, matrix if negative
What is the name for the electrochemical gradient across the inner mitochondrial membrane?
The proton-motive force (pmf)
What does the proton motive force drive?
ATP synthesis
What is the relative pH in the intermembrane space of the mitochondria?
low PH, due to increased [H+]
What is the relative pH in the mitochondrial matrix?
High pH, due to protons being pumped out and therefore a low [H+]
What is a bacteriorhodopsin?
A light induced proton pump that uses energy from light to make a proton gradient across a lipid vesicle. This facilities ATP synthase prodcution of ATP
What is the impact of DNP on oxidative phosphorylation?
Shuttles H+ back from inter-membrane space to the matrix, dissipating the proton gradient and preventing ATP synthesis. Energy builds up and is dissipated as heat
What are the 2 parts of ATP synthase?
F1 and FO
Where is F1 of F1FO-ATP synthase found?
In the mitochondrial matrix
Where is F1 of F1FO-ATP synthase found?
In the inner mitochondrial membrane
What are the 2 types of subunits in ATP synthase, as characteristed by their ability to turn?
Rotor and stator subunits
What components of ATP synthase are rotor subunits?
c subunits, gamma stalk, epsilon
What components of ATP synthase are stator subunits?
a,b, alpha and beta subunits
