Lecture 3 Flashcards
Methods of propagation
Seed propagation
Vegetative propagation
Tissue & cell culture
Cell and tissue culture
We take apart the plant that contains all genetic information (buds ) provide it with specific regimen of nutrients, hormones, light under serial condition in laboratory
Seed propagation
In gallery
Vegetative propagation
Cutting
Division
Layering
Grafting
Budding
Cutting
Mostly widely used method
Cutting plant portion capable to develop roots (have buds )
Rooting media should be enriched with hormones
Division
The separation of plant with number of buds or aerial stems into separate parts each having root and growing points
Layering
A method of propagation by which stem or shoot of a plant is induced to produce root while being attached to the parents plants
Grafting
نباتين مختلفين بس قريبين من بعض ينقطع حته من النبات و نحط عليه النبات التاني اللي عايزين نزودوا
Portion of variety to be increased (secion) chosen root system (stock)
So 2 become one living unit
Budding
Modified from grafting
Introducing a piece of bark bearing buds (scion) suitable cavity T-shaped slit made in the bark of stock
Totipotency
The ability of plant cell to develop into a fully differentiated
plant. This is because every cell has the genetic potential of
the parent plant
Meristematic cells
are
the
undifferentiated cells at shoot and root
tips. They have the greatest potential to
produce cells that will become shoots or
roots.
▪The explant
can be part of any organ,
tissue or cells (shoot, leaves, roots,
flower or cells ) from a plan
Explants to Callus
Explants are then usually placed on the surface of a solid
culture medium, but are sometimes placed directly into a
liquid medium, when cell suspension cultures are desired.
