Lecture 17- Bacterial Classification & Morphology Flashcards

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1
Q

In what way did Avon Leeuwenhoek I rove the microscope and when did he first see microorganisms?

A

Made microscope clearer up to 200x, in 1675 he saw the microorganisms

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2
Q

What is taxonomy?

A

The identification and naming of organisms

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3
Q

How is the binomial naming system derived?

A

[Genus descriptive feature]= Species

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4
Q

What is panther leo Panthers onca Panthers pardus?

A

panther leo = Lion Panthers onca= jaguar Panthers pardus= leopard

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5
Q

How many original kingdoms were there (name them) and how many are there now?

A

2 (plants and animals) now 6

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6
Q

Is it true that the division between prokaryotes and Eukaryotes is based on Kingdom?

A

No, because while bacteria and Archaea are both prokaryotes, they are divided into 2 different domains.

instead the difference between prokaryotes and Eukaryotes is cell structure and organization

Prokaryotes: organisms without membrane bound organelles and nucleus

opposite for Eukaryotes

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7
Q

What is the typical range of length and width of prokaryotes?

A
  • 1-10 μm length
  • 0.7-1.5μm width
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8
Q

What are difference in characteristic between Bacteria and Archaea?

A
  • Archaea do not have peptidoglycan cell wall
  • Archaes have 3 types of RNA polymerase (R,T,M) while Bacteria 1
  • the protein synthesis imitator in bacteria is f-Methionine , archaea Methionine
  • growth is inhibited for bacteria in presence of Streptomycin (antibiotic) Archaea not
  • DNA in bacteria is not associated with histones, Archaea it is
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9
Q

What is the characteristic differences between Archaea and Eukaryotes

A
  • Archaea have no nuclear envelopes & membrane enclosed organelles ​Eukaryotes ​do
  • Archaea chromosomes are circular while Eukaryotes linear
  • Archaea 70S Eukaryotes 80S
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10
Q

What Is the practical use of classification?

A

identification (isolating and identifying disease causing agents)

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11
Q

What is the conventional classification scheme?

A

classification on visible phenotypic features (similarities and differences)

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12
Q

what are microbes grouped according to in molecular classification?

A

Evolutionary relatedness

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13
Q

What are some conventional features used to classify bacteria?

A
  • Cell wall
  • shape of bacteria
  • Bacterial size
    presence or absence of flagella
  • presence or absence of pili
  • presence or absence of capsule
  • ability to form spores
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14
Q

What are characteristics of gram-positive bacteria?

A
  • have a thick peptidoglycan layer
  • 1 membrane bilayer
  • lower lipid content compared to -
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15
Q

What are some ways cocci (round bacteria) can arrange?

A
  • pairs
  • chains
  • clusters
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16
Q

What are the ways that bacilli (rod) shaped bacteria can rearrange, and are all of them sport forming?

A
  • regular/irregular
  • No, some are spore forming, some are not
17
Q

What would happen if you performed gram staining on Mycoplsma, and are they considered + or -?

A

they would not stain (no cell wall) however they are considered gram + because of molecular classification

18
Q

What is the unique feature of acid fast bacteria, and where are they included according to molecular classification

A
  • they do not ​stain well. However they hold their stain if they do stain
  • sre considered gram + according to molecular classification
19
Q

What are the two main genera of acid-fast bacteria?

A
  • Mycobacterium (have split into several genres)
  • Nocardia
20
Q

What are some unique features of gram - ​bacteria?

A
  • have a thin peptidoglycan cell wall (sandwiched)
  • 2 bilayers
  • contain lipopolysaccharide on outer cell membrane
  • Some are obligate intracellular parasites
  1. Rickettsia (have cell wall)
  2. Chlamydia (no cell wall)
21
Q

What is molecular classification and how is it used?

A

Identifying similarities and differences of organisms according to evolutionary relatedness

this is assessed by comparing DNA sequences to useful genes (common in many organisms and not too closely related with all organisms or too far)

Eg- Small subunit (SSU) of rRNA Gene sequences & others

22
Q

How many kingdoms make up Eukaryotes?

A

4