Lecture 14

Question 1

Transcriptional control includes

Answer 1

core promoters and regulatory sequences/motifs

Question 2

Core promoters are important for

Answer 2

recruitment of RNA pol II

Question 3

core promoter elements

Answer 3

TATA, BRE, TSS, DPE

Question 4

regulatory sequences are

Answer 4

binding motifs that attract or recruit TF’s

Question 5

do elements bind directly to core promoter?

Answer 5

no, only onto TBP or TATA binding protein

Question 6

gene regulatory elements

Answer 6

enhancer
silencer
insulator
activator
SWI/SNF complex
mediator complex
general TF’s

Question 7

Distal regulatory elements steps

Answer 7

1. Activators bind to enhancers, causing bend
2. Activators work with co-activators which causes chromatin remodeling via SWI/SNF complex and histone trans-acetylase
3. Activators bind to Mediator complex then attracts the enhancer to the core promoter, causing creation of RNA pol and gen TF’s at promoter site