Lecture 13

Question 1

what is the idea of genomic equivalence

Answer 1

all cells carry master blueprint, meaning a single cell from an organism can create a copy of the organism

Question 2

examples of genomic equivalence

Answer 2

carrot
dolly they sheep

Question 3

Types of cells

Answer 3

totipoten, pluripotent, multipotent

Question 4

totipotent cells are

Answer 4

fertilized eggs

Question 5

pluripotent cells are

Answer 5

early embryonic cells

Question 6

multipotent cells are

Answer 6

not fully differentiated cells, stem cells

Question 7

pluripotent stem cells can be

Answer 7

harvested or made

Question 8

TF’s involved in making pluripotent stem cells

Answer 8

Oct4
Sox2
Klf4
C-Myc

Question 9

How do the TF’s work together to make pluripotent stem cells?

Answer 9

they decondensed chromatin, expose DNA in promoter regions, and remove mods to the DNA making it a “clean slate”

Question 10

DNAase I does what

Answer 10

detects transcriptionally active chromatin, showing which cells are being used

Question 11

what can DNAase I do for researchers

Answer 11

cut up chromatin when cell is dying, apotosis

Question 12

DNA methylation is

Answer 12

methyl group added to cytosine to CpG site

Question 13

what does DNA methylation do

Answer 13

blocks gene expression

Question 14

where are CpG sites concentrated

Answer 14

at promoter regions to regulate gene expression

Question 15

X-inactivation causes what

Answer 15

x-ist RNA results in condensation into Barr Body

Question 16

X-inactivation allows

Answer 16

limit of gene expression