Lecture 13 Notes Flashcards

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1
Q

oligonucleotide primer (20 nucleotides)

A

the primer defines a fixed, common reference point for DNA strands synthesized in the sequencing reaction

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2
Q

next gen sequencing

A
  • various high throughput methods used to sequence random fragments of DNA without the need for making recombinant DNA clones
  • genomic DNA randomly fragmented by shearing
  • linker/adaptors ligated to ends
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3
Q

human genome project

A
  • human genome is 3 billion base pairs
  • major accomplishments: DNA sequence of entire genome = RefSeq; large fragments of genome isolated and organized; DNA sequence annotated to define genes; DNA sequences of model organisms and other species
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4
Q

Genome projects 1

A

determining sequence of human genome

  • fragmenting the genome
  • sequencing the genome
  • reconstructing the genome sequence from fragments
  • finding the genes
  • what the genome looks like
  • bioinformatics tools
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5
Q

fragmenting the genome

A
  • cut genomic DNA into fragments of different average sizes
  • separate genomic DNA fragments into a particular size class
  • insert the DNA fragments into cloning vectors to generate libraries
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6
Q

paired end sequencing

A

preferred method for sequencing eukaryotic genomes for the first time

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