Lecture 12: Intracellular Bacteria Flashcards
What is a facultative intracellular pathogen?
Can divide independently and on agar plates but also can enter host cells as part of their pathogeesis
What is an obligate intracellular pathogen?
require host cell resources to multiply
What are examples of facultative intracellular pathogens?
Neisseria, Enterics, Mycobacter, Bacilli, Legionella
What are examples of obligate intracellular pathogens?
Rickettsial, Chalmydia
How are obligate intracellular pathogens grown in lab?
In tissue culture
like viruses
How are intracellular pathogens transported around the human body?
In macrophages…sneaky
How do intracellular pathogens evade humoral and surface-innate immunity (actin-based cell-to-cell spread)?
They’re in macrophages
What are 2 functions of T3SS for intracellular pathogens?
Enhance phagocytosis by the target cell type
Alter endosome so lysosomes fail to fuse to it
What must antibiotics do to treat intracellular pathogens? What class of drug is used?
Cross human cell membrane and remain active/available to do so
Tetracyclines
What is a potential downfall to using tetracyclines to treat intracellular pathogens?
contraindicated during pregnancy
How is Listeria monocytogenes classified?
facultative intracellular
What does Listeria monocytogenes cause, and what does it contaminate for transmission to humans?
causes gastroenteritis
contaminates cold-stored pre-prepared foods
Why do pregnant woman avoid cheeses and deli meat during pregnancy?
Listeria monocytogenes
What can Listeria monocytogenes cause in immunosuppressed people?
meningitis in addition to gastroenteritis
How does Listeria monocytogenes move in the human body?
Endocytosis
escapes endosome
uses actin-based motility to spread between cells
