Lecture #10-12 = Enzyme kinetics, inhibition and regulation

Question 1

What is a progress curve and what does its decrease in gradient relate to?

Answer 1

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Question 2

What is Vo proportional to when substrate is in excess?

Answer 2

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Question 3

Where on the graph of V vs S do you find first order and second order kinetics?

Answer 3

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Question 4

What two important values can be calculated from the V vs S curve? (hyperbolic)

Answer 4

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Question 5

What is the equation of the hyperbolic curve and why is it famous?

Answer 5

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Question 6

What’s another name of Km?

Answer 6

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Question 7

What are the four assumptions that the Michaelis-Menten equation is based on?

Answer 7

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Question 8

How (in a lab) would you measure the Km and Vmax? And since that hyperbolic graph is impractical, what would you do? Explain that equation and its components

Answer 8

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