lect 6- bact. genome replication, expression and reg 2 Flashcards
define global regulation
regulatory systems that affect many genes and pathways simultaneously
define regulon
genes or operons controlled by common regulatory protein
define modulon
operon network under control of common global regulatory protein but individual operons are controlled separately by their own regulators
define activator
enhances transcription needed
define repressor
inhibits transcription unless needed
what are the 2 proteins involved in 2 component regulatory system
sensory kinase and response-regulator protein
define EnvZ
sensory kinase: autophosphorylates in high osmolarity
define OmpR
response regulator: phosphorlyated and regulates transcription
what does sigma factor 70 do
transcribes genes that are needed during exponential growth
what does CheY do
governs rotation of flagella
is CheA.CheW active/phosphorylated when attractant concentration is high or low
when it is high
what does LuxR do
stimulates transcription of the genes for AHL synthase (luxe) and proteins needed for light production
define auxotrophic mutant
unable to make an essential macromolecule such as an aa or nucleotide
what corrects errors in DNA/does proofreading
DNA polymerase
what is photoreactivaiton direct repair used for
to directly rem air thymine dimers
define exogenote
DNA that is transferred to recipient
define endogenote
genome of recipient
define merozygote
recipient cell that is temporarily diploid as result of transfer process (HGT)
what are the 3 types of recombination at the molecular level
homologous recombination, site specific recombination, transposition
what carries out the process of homologous recombination
RecA
what is site specific recombination mediated by
recombinase enzymes
define composite transposons
transposable elements which contain extra genes
what do F factors contain
info for formation of sex pilus
what do the insertion sequences of F factors do
assist in plasmid integration
