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ClinChem2 3rdYR 1stSem Prelims > (LEC) Amylase & Lipase > Flashcards

(LEC) Amylase & Lipase Flashcards

1
Q

EC of Amylase

A

EC 3.2.1.1

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2
Q

Other name for Amylase

A

1,4-D-Glucan Glucanohydrolase

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3
Q

Molecular weight of Amylase

A

50-55 kDa

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4
Q

Enzyme the catalyzes the breakdown of starch and glycogen

A

Hydrolase

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5
Q

Consists of both Amylose & Amylopectin

A

Starch

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6
Q

chain of glucose linked by a-1,4-glycosidic bond

A

Amylose

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7
Q

chain of polysaccharide linked by a-1,6-glycosidic bond

A

Amylopectin

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8
Q

Cofactors for Amylase

A

Ca2+ & Cl-

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9
Q

Major Sources of Amylase

A

Salivary Glands
Pancreas

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10
Q

Primary Isoenzymes of Amylase

A

S-isoamylase
P-isoamylase

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11
Q

Reference Range of Serum Amylase

A

28-100 U/L

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12
Q

Reference Range of Urine Amylase

A

1-15 U/h

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13
Q

1 Somogyi Unit = ?

A

1.85 IU

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14
Q

Clinical Correlation with Elevated Amylose Levels

A

Acute Pancreatitis
Salivary Gland Disease
Intra-abdominal Disease

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15
Q

Condition when an amylase molecule combines with IgG or IgA or a polysaccharide to perform a complex that is too large to be filtered across the renal glomerulus

A

Macroamylasemia

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16
Q

Amylase Isoenzyme that migrates quickly in Electrophoresis

A

S-amylase

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17
Q

Amylase Isoenzyme that migrates slower in Electrophoresis

A

P-amylase

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18
Q

Most commonly observed fractions in Amylase Electrophoresis

A

P2, S1, S2

19
Q

Predominant amylase isoenzyme in acute pancreatitis but not specific

A

P3

20
Q

Amylase Assay:

Measures the disappearance of starch substrate

A

Amyloclastic

21
Q

Amylase Assay:

Measures the appearance of the product

A

Saccharogenic

22
Q

Amylase Assay:
Measures increasing color from production of product coupled with a chromogenic dye

A

Chromogenic

23
Q

Amylase Assay:

Coupling of several enzyme systems to monitor amylase activity

A

Continuous Monitoring

24
Q

In Amyloclastic Method, ______ is attached to starch substrate forming dark blue color

A

Iodine

25
Q

Amylase Assay:

Uses starch substrate that is hydrolyzed by amylase to its constituent carbohydrate molecules that have reducing properties

A

Saccharogenic Method

26
Q

Amylase Assay:

Amylase hydrolyzes starch substrates, forming smaller water soluble dye substrate fragments resulting to increase in color intensity

A

Chromogenic Method

27
Q

Coupling Enzymes used in Continuous Monitoring Method

A

A-glucosidae
Hexokinase
G-6-P dehydrogenase

28
Q

Optimal pH in Continuous Monitoring Method

A

pH 6.9

29
Q

Preferentially inhibits salivary amylase

A

Wheat Germ Lectin

30
Q

Can suppress or inhibit amylase activity

A

Plasma Triglyceride

31
Q

Falsely elevates serum amylase

A

Morphine / Opioids

32
Q

Anticoagulant that should be used for Amylase Assays

A

Heparin

33
Q

EC of Lipase

A

EC 3.1.1.3

34
Q

Other name of Lipase

A

Triacylglycerol Acylhydrolase

35
Q

Molecular Weight of Lipase

A

45 kDa

36
Q

Major and Primary source of serum lipase

A

Pancreas

37
Q

Most clinically specific and sensitive Lipase Isoenzyme

A

L2

38
Q

Reference range for Serum Lipase

A

< 38 U/L

39
Q

Clinical Correlation of Elevated Lipase levels

A

Acute Pancreatitis
Renal Disorder
Humps virus

40
Q

Lipase Assay:

Estimation of liberated fatty acids and turbidimetric method

A

Titrimetric method

41
Q

Substrate for Classic Cherry-Crandall Method

A

Olive Oil

42
Q

Substrate for Modified Cherry-Crandall Method

A

Triolein

43
Q

Lipase Assay:

Based on coupled reaction with Coupling Enzymes such as
- Glycerol Kinase
- Glycerophosphate Oxidase
- Peroxidase

A

Colorimetric Method

44
Q

Causes falsely low Lipase levels

A

Hemolysis

ClinChem2 3rdYR 1stSem Prelims (12 decks)

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