(LAB) ALP and ACP Flashcards
E.C. No. of ALP
E.C. 3.1.3.1
Systematic Name of ALP
Orthophosphoric Monoester Phosphohydrolase (Alkaline Optimum)
Isoenzyme ALP
Placental ALP
Bone ALP
Intestinal ALP
Liver ALP
ALP Isoenzyme elevated in normal pregnancy
Placental ALP
ALP Isoenzyme elevated in Blood type B and O individuals
Intestinal ALP
ALP Isoenzyme elevated children or growing individuals
Bone ALP
Other Isoenzymes of ALP (Abnormal Fractions)
Regan
Nagao
Kasahara
Substrate of ALP
Phosphomonoester
Product of ALP
Alcohol + Phosphate ion
ALP Optimal pH
9.0 - 10.0
ALP Activators
Mg2+ and Zn2+
Most commonly used method Continuous-Monitoring Technique for ALP Assay
Bessey-Lowry and Brock method
6 Methods of ALP Measurement
- Shinowara
- King and Armstrong
- Bessey-Lowry and Brock
- Huggins and Talalay
- Moss
- Klein, Babson, and Read
Substrates of ALP Measurement Methods (In order based on table)
- Beta-glycerophosphate
- Phenyl Phosphate
- Para-Nitrophenyl phosphate
- Phenolphthalein Diphosphate
- Alpha-Naphthol Phosphate
- Buffered Phenolphthalein Phosphate
End Products of ALP Measurement Methods (In order based on table)
- Inorganic Phosphate + glycerol
- Phenol
- Para-Nitrophenol
- Phenolphthalein red
- Alpha-Naphthol
- Free Phenolphthalein
Acidic blood when measure ALP results
Falsely low
ALP reference range for women
35 - 104 U/L
ALP reference range for Men
40 -129 U/L
ALP reference range for Children
54 - 369 U/L
E.C. of ACP
E.C. 3.1.3.2
Systemic Name of ACP
Orthophosphoric Monoester Phosphohydrolase (Acid Optimum)
ACP Substrate
Phosphomonoester
ASP Products
Alcohol & Phosphate Ion
Substrate of choice for quantitative endpoint reaction
Thymolphthalein Monophosphate
Substrate of choice for continuous monitoring methods
Alpha-Naphthyl Phosphate
Richest Gland with ACP
Prostate Gland
4 Methods of ACP Measurement
- Gutman and Gutman
- Shinowara
- Bason, Read, and Philips
- Ray and Hillman
Substrates of ACP Measurement Methods (In order based on table)
- Phenyl Phosphate
- Para-Nitrophenyl Phosphate
- Alpha-Naphthyl Phosphate
- Thymolphthalein Monophosphate
End Products of ACP Measurement Methods (In order based on table)
- Inorganic Phosphate
- Para-Nitrophenol
- Alpha-Naphthyl
- Free Thymolphthalein
Substrate of Choice for Kinetic Method
Babson, Read, and Philips
Substrate of Choice for End Point Reaction
Ray and Hillman
Tissue sources of ACP that may cause false results
RBCs and Platelets
When will ACP activity decrease when left at Room Temp
Within 1-2hrs
Reference range of ACP for Men at 25C
3.6
Reference range of ACP for Men at 30C
5.0
Reference range of ACP for Men at 37C
6.5
Reference range of ACP for Women at 25C
3.0
Reference range of ACP for Women at 30C
4.2
Reference range of ACP for Women at 37C
5.5