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ClinChem2 3rdYR 1stSem Prelims > (LAB) Pancreatic Enzymes > Flashcards

(LAB) Pancreatic Enzymes Flashcards

1
Q

Lipase is also known as

A

Triacylglycerol Acylhydrolase

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2
Q

Lipase catalyzes TAG in the intestines into what

A

2-monoglyceride & fatty acids

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3
Q

Optimal Temp for Lipase Assay

A

40 C

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4
Q

Optimum pH for Lipase Assay

A

8.8

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5
Q
A
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6
Q

Lipase Assay Method:

Measures the liberated fatty acids by titration after a 24hr incubation

A

Classic Cherry-Crandall Method

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6
Q

Lipase Assay Method:

Based on the coupled reactions with enzymes

A

Colorimetric method

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6
Q

Substrate used for Classic Cherry-Crandall Method

A

Olive Oil

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6
Q

Pancreatic Enzymes that are stable in Serum

A

Lipase & Amylase

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6
Q

Lipase Assay Method:

Dispersed particles of hydrolyzed fats and the rate of clearing can be measured as an estimation of LPS activity

A

Turbidimetric Method

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6
Q

Enzymes with coupled reaction in the Colorimetric Method

A

Peroxidase
Glycerol Kinase

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6
Q

Modified substrate for Classic Cherry-Crandall Method

A

Triolein

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6
Q

What conditions do not interfere with Turbidimetric Method

A

Icterus
Lipemia
Hemolysis

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7
Q

Pancreatic Enzymes unstable in Serum

A

Lactate Dehydrogenase

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8
Q

In room temperature, how long will Lipase activity last before a loss

A

1 week

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9
Q

In 4 C, how long will Lipase activity last before a loss

A

at least 3 weeks

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10
Q

Why should Hemolysis be avoided in Lipase Assays

A

Hemoglobin inhibits serum in Lipase

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11
Q

Reference range of Lipase in Room Temperature

A

0-1.0 U/L

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12
Q

Reference range of Lipase at 37 C

A

< 38 U/L

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13
Q

Enzyme Commission Number of Lipase

A

E.C. 3.1.1.3

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14
Q

Enzyme Commission Number of Amylase

A

E.C. 3.2.1.1

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15
Q

Amylase is aka

A

1,4 - D - Glucan Glucanohydrolase

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16
Q

This Enzyme breaks down starch and glycogen to monosaccharides

A

Amylase

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17
Q

Activators of Amylase

A

Calcium & Chloride ions

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18
Q

Amylase found in Humans

A

Alpha Amylase

19
Q

Amylase found in Plants

A

Beta Amylase

20
Q

Amylase Assay Method:

AMS hydrolyzes starch, the Iodine is released and a decrease in the initial dark blue color intensity of the starch-iodine complex occurs.

A

Amyloclastic Method

21
Q

In Amyloclastic Method, if there is a decrease in the Dark Blue Color Intensity, what does this mean for the AMS concentration?

A

Increase in AMS concentration

22
Q

Amylase Assay Method:

The amount of reducing sugars is measured where the concentration is proportional to AMS Activity

A

Saccharogenic Method

23
Q

Classic Reference method for Saccharogenic Method

A

Sumogyi Units

24
Q

Amylase Assay Method:

As AMS hydrolyzes starch substrate, smaller dye substrate fragments are produced, and these are water soluble

A

Chromogenic Method

25
Q

In the Chromogenic Method, if there is an increase in Color Intensity, what does this mean for the AMS Concentration?

A

Increase in AMS Concentration

26
Q

Amylase Assay Method:

Have been used to determine AMS activity by a continuous-monitoring technique in which the change in absorbance of NAD+ at 340 nm is measured

A

Coupled-Enzymes

27
Q

Difference between Coenzyme and Activator

A

Coenzyme - uses Organic substances
Activator - uses Inorganic substances

28
Q

Amylase Assay Method:

Salivary and pancreatic amylase can be estimated by measuring total AMS in the presence and absence of lectin

A

Wheat Germ Lectin

29
Q

This amylase assay method inhibits salivary amylase

A

Wheat Germ Lectin

30
Q

Loss activity for Amylase in room temperature occurs when

A

1 week

31
Q

Loss activity for Amylase at 4 C occurs when

A

2 - 6 months

32
Q

Falsely elevates serum AMS levels

A

Morphine (Opiates)

33
Q

Falsely lowers AMS activity

A

Anticoagulants:
Citrate
EDTA
Oxalate

34
Q

Why is hemolysis not a problem in Amylase Assays

A

RBCS dont contains Amylase

35
Q

Reference range of Amylase in Serum at 37 C

A

28 - 100 U/L

36
Q

Reference range of Amylase in Urine

A

1 - 15 U/h

37
Q

Enzyme Commission Number of Lactate Dehydrogenase

A

E.C. 1.1.1.27

38
Q

This enzyme catalyzes the interconversion of lactic and pyruvic acid

A

Lactate Dehydrogenase

39
Q

4 subunits of Lactate Dehydrogenase

A

LD-1 HHHH
LD-2 HHHM
LD-3 HHMM
LD-4 HMMM
LD-5 MMMM

40
Q

Normal LD pattern

A

2 1 3 4 5

41
Q

Flipped LD pattern

A

1 2 3 4 5

42
Q

Causes of Flipped LD pattern

A

Myocardial Infarction
Anemia
Hemolysis

43
Q

Substrate used for Electrophoresis in Lactate Dehydrogenase Assay

A

Alpha-hydroxybutyrate

44
Q

Optimal pH of Forward Reaction in LD Electrophoresis

A

8.3 - 8.9

45
Q

Optimal pH of Reverse Reaction in LD Electrophoresis

A

7.1 - 7.4

46
Q

Lactate Dehydrogenase in Serum should be analyzed within how many hours?

A

48hrs

47
Q

Most labile subunit of Lactate Dehydrogenase

A

LD-5 (MMMM)

48
Q

LD isoenzyme analysis should be analyzed within how many hours?

A

24hrs

49
Q

What anticoagulant does not interfere with AMS

A

Heparin

50
Q

Ratio of anticoagulant in Blacktop (Differentiates it from Light Blue top)

A

1:4

51
Q

Another name for the Forward Reaction in the Electrophoresis of Lactate Dehydrogenase

A

Wacker Method

52
Q

Volume of Working Reagent

A

1000 uL

53
Q

Volume of sample (LD , AMS)

A

10uL , 25 uL

54
Q

Provided factor for Lactate Dehydrogenase estimation

A

16030

55
Q

Provided factor of Amylase estimation

A

3178

ClinChem2 3rdYR 1stSem Prelims (12 decks)

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