LE3-Micro Flashcards
- Which of the following specimens for culture of Salmonella typhi will have the highest sensitivity?
a. Urine
b. Blood
c. Intestinal secretions
d. Bone marrow
d. Bone marrow
Bone Marrow: Culturing from bone marrow is considered the gold standard for diagnosing typhoid fever caused by Salmonella typhi. It remains the most sensitive method, even in patients who have already started antibiotic therapy.
- Swarming growth on blood agar
a. E. coli
b. Proteus mirabilis
c. Pseudomonas aeruginosa
d. Shigella dysenteriae
e. Serratia marcescens
b. Proteus mirabilis
Proteus mirabilis: Known for its swarming growth pattern, where it rapidly spreads across the surface of the agar plate.
- Medium-sized colonies that appear buff in color on blood agar
a. E. coli
b. Proteus mirabilis
c. Pseudomonas aeruginosa
d. Shigella dysenteriae
e. Serratia marcescens
d. Shigella dysenteriae
Shigella species can form medium-sized colonies on blood agar, which may appear as a pale, translucent, or buff color.
- Non-motile; no gas from dextrose; colorless and transparent
a. E. coli
b. Proteus mirabilis
c. Pseudomonas aeruginosa
d. Shigella dysenteriae
e. Serratia marcescens
d. Shigella dysenteriae
Non-motile, does not produce gas from dextrose, and can form colorless, transparent colonies on certain media.
- Metallic sheen on differential media
a. E. coli
b. Proteus mirabilis
c. Pseudomonas aeruginosa
d. Shigella dysenteriae
e. Serratia marcescens
a. E. coli
Known for producing a metallic sheen on EMB agar, which is a result of the strong acid production from lactose fermentation.
- Soluble pigments; blue green and fluorescing
a. E. coli
b. Proteus mirabilis
c. Pseudomonas aeruginosa
d. Shigella dysenteriae
e. Serratia marcescens
c. Pseudomonas aeruginosa
Characteristically produces blue-green pigments (pyocyanin and pyoverdine) and exhibits fluorescence under UV light.
- This organism has been described as an infrequent cause of nosocomial infections of the urinary tract and wounds and rare cases of bacteremia.
A. P.mirabilis
B. S.marcescens
C. P.retgerri
D. M.morgani
B. S.marcescens
S. marcescens (Serratia marcescens): Known for causing nosocomial infections, including urinary tract infections, wound infections, and, less frequently, bacteremia.
- This enteric bacteria can produce lobar pneumonia with extensive hemorrhagic necrotizing consolidation of the lung which produces “currant jelly” sputum from hemoptysis and abscess formation.
A. Klebsiella sp.
B. Enterobacteria sp.
C. Proteus sp.
D. Serratia sp.
A. Klebsiella sp.
Klebsiella pneumoniae, a species within the Klebsiella genus, is well-known for causing severe, aggressive forms of pneumonia, particularly in immunocompromised individuals. The “currant jelly” sputum is a classic clinical feature associated with Klebsiella pneumonia, resulting from the combination of blood (hemoptysis) and mucoid material.
19 This bacteria is Gram positive, endospore forming anaerobe found in the stool of many healthy adults, infection is usually precipitated by the extended use of antibiotics especially fluroquinolones
A. Clostridium difficile
B. Bacillus cereus
C. Vibrio cholerae
D. Campylobacter
A. Clostridium difficile
Clostridium difficile (C. difficile): This bacterium is a well-known cause of antibiotic-associated diarrhea and more serious intestinal conditions such as pseudomembranous colitis. The disruption of normal gut flora by antibiotics, particularly broad-spectrum antibiotics like fluoroquinolones, can lead to an overgrowth of C. difficile.
- This following is true of the antigenic structure of enterobacteriaceae
a. antibodies to O antigens are predominantly IgG
b. H antigens may intefere with agglutination by O antisera and they may be associated with virulence
c. H antigens are located on flagella and are denatured or removed by heat or alcohol
d. O antigens are susceptible to heat and alcohol and usually are detected by bacterial agglutination
c. H antigens are located on flagella and are denatured or removed by heat or alcohol
- Which of the following shigella specie will present a positive ornithine carboxylase
A. S. Flexneri
B. S. Dysenteriae
C. S. Sonnei
D. S. Boydii
C. S. Sonnei
- Enteric bacteria can be characterized by the following, except
a. gram negative rod
b facultative anaerobes and aerobes
c. catalase negative, oxidase negative
d. ferment rather than oxidize glucose
c. catalase negative, oxidase negative
Most enteric bacteria are catalase (+) positive and oxidase (-) negative.
The catalase test differentiates between bacteria that can produce the enzyme catalase, which breaks down hydrogen peroxide. The oxidase test is used to identify bacteria that produce certain cytochrome c oxidases. Enteric bacteria, being part of the Enterobacteriaceae family, are typically oxidase negative but catalase positive.
- The medium is often used to help differentiate salmonella and shigellae from other enteric gram-negative rods in stool cultures
A. Mannitol salt agar
B. Triple Sugar iron agar
C. MacConkey agar
D. Eosin Methylene blue
C. MacConkey agar
- This antigenic structure of enteric bacteria is the most external part of the cell wall lipopolysaccharide and consist of repeating units of polysaccharide.
A. VI antigen
B. K antigen
C. O antigen
D. H antigen
C. O antigen
- the bacteria is a comma shaped, cuved rod 2-4mm long, actively motile by means of polar flagellum
A. camptylobacter
B. escherichia coli
C. clostridium difficile
D. vibrio cholerae
D. vibrio cholerae
Vibrio cholerae is known for its distinctive comma shape, and it is motile with a single polar flagellum.
- The antibiotic preferred for the treatment of non-thypoidal salmonella gastroenteritis is
a tetracycline
b none
c. fluoroquinolones
d amoxicillin
b none
This is often the correct approach for otherwise healthy individuals with non-typhoidal Salmonella gastroenteritis, as the infection is usually self-limiting.
- Which of the following specimens for culture of Salmonella typhi will have the highest sensitivity?
A. Urine
B. Blood
C. Intestinal secretions
D. Bone marrow
D. Bone marrow
- A 24 year old patient came in with flank pain, dsyuria, and urinary frequency for 5 days. Patient described the smell of his urine as similar to that of ammonia. Which organism is most likely causing the patient’s condition?
A. Morganella morganii
B. Salmonella typhi
C. Escherichia coli
D. Proteus mirabilis
D. Proteus mirabilis
This organism is known for causing UTIs and is particularly noted for producing a strong ammonia smell in urine due to its ability to break down urea into ammonia. It is also associated with the formation of kidney stones and can cause flank pain.
- The following is/are true of the possible TSI reaction of Proteus mirabilis?
A. alkaline slant, alkaline butt, (-) gas, (-) H2S
B. alkaline slant, acid butt, (-) gas, (-) H2S
C. alkaline slant, acid butt, (-) gas, (+) H2S
D. acid slant, acid butt, (+) gas, (-) H2S
C. alkaline slant, acid butt, (-) gas, (+) H2S
- Which Proteus species would have Spot indole test positive result?
A. Proteus myxofaciens
B. Proteus vulgaris
C. Proteus mirabilis
D. Proteus penneri
B. Proteus vulgaris
Proteus vulgaris is known to be indole positive.
- The specie of shigella that does not ferment mannitol
a. S. dysenteriae
b. S. sonnei
c. S. flexneri
d. S. boydii
a. S. dysenteriae
Does not ferment mannitol. This species is known for causing severe forms of dysentery.
- The shigella exotoxin may contribute to the extreme severity and fatal infections of the bacteria and to the central nervous system reactions observed in them. This activity is called?
A. Neurotoxic
B. endotoxic
C. antitoxin
D. enterotoxin
A. Neurotoxic
This term refers to toxins that are harmful or destructive to nerve tissue (neuro-). The shigella exotoxin can affect the nervous system, leading to symptoms such as seizures, especially in children, which aligns with the description of neurotoxic effects.
- the primary virulence factor in these bacteria is shiga toxin
a. enteroinvasive e coli
b enterohemorrhagic e coli
c. enterpathogenic e coli
d enteroaggregative e coli
b Enterohemorrhagic e coli
EHEC, particularly the O157:H7 strain, is known for producing Shiga toxin, which is responsible for causing severe gastrointestinal symptoms, including bloody diarrhea, and can lead to hemolytic uremic syndrome (HUS), a serious complication affecting the kidneys.
- A 24 year old student came in with high grade fever, weakness and diarrhea. upon examination, blanching macules were seen in the chest and the abdomen. the most likely organism associated with these signs and symptoms is
a vibrio cholerae
b escherichia coli
d salmonella typhi
d shigella dysenteria
d salmonella typhi
Salmonella typhi causes typhoid fever, which is characterized by prolonged high-grade fever, weakness, gastrointestinal symptoms, and rose spots on the lower chest and abdomen.
65 Major cause of diarrhea in infants characterised as severe and watery with vomiting and fever are usually self-limited but can be prolonged or chronic
A. Enterinvasive E. coli
B. Enterehemorrhagic E. coli
C. Enteroaggregative E. coli
D. Enteropathogenic E. coli
D. Enteropathogenic E. coli
EPEC is a significant cause of watery diarrhea in infants, especially in developing countries. It’s known for causing a range of symptoms, including severe, watery diarrhea and vomiting, and can be self-limited or prolonged.
- The bacteria is a large, gram positive, endospore-forming bacterium found in soil and vegetation, common in rice dishes; wherein heating the food does not always kill the spores, which germinate as the food cools
A. Vibrio cholerae
B. Bacillius cereus
C. Campylobacter
D. Clostridium difficile
B. Bacillius cereus
Bacillus cereus is a Gram-positive, spore-forming bacterium commonly found in soil. It is known for causing food poisoning, particularly from rice dishes. The spores can survive cooking and germinate as the food cools, leading to toxin production and foodborne illness.
- What do you call the capsular antigen of Salmonella typhi?
A. K antigen
B. O antigen
C. H antigen
D. VI antigen
D. VI antigen
VI antigen is the capsular antigen specific to Salmonella typhi, playing a role in its virulence and immune evasion.
- The growth habit of this category of e. coli cause a “stacked brick” configuration on tissue culture cells
a. enteroaggregative e. coli
b enterohemorrhagic e. coli
c. enteropathogenic e. coli
d. enteroinvasive e. coli
a. Enteroaggregative e. coli
EAEC is known for its distinctive “stacked brick” adherence pattern on epithelial cells. This adherence pattern is associated with persistent diarrhea, particularly in children in developing countries.
- The most common cause of bacterial meningitis in the newborn
A. S. aureus
B. E. coli
C. S. pneumonia
D. N. meningitidis
B. E. coli
B. E. coli (Escherichia coli): This is the correct answer. E. coli, particularly certain strains like K1, is a leading cause of neonatal meningitis. Newborns are particularly susceptible to E. coli infections, which can be severe and include meningitis.
- This gram (-) enteric bacteria do not ferment lactose, no gas from dextrose and is non-motile
A. E. coli
B. Proteus Species
C. Shigella
D. Salmonella Species
C. Shigella
Does not ferment lactose, does not produce gas from dextrose, and is non-motile.
- one of the common pathogen associated with travellers diarrhea
a. enterohemorrhagic e. coli
b enteropathogenic e coli
c enteroinvasive e coli
d enteroaggregative e coli
d Enteroaggregative E.coli
EAEC is increasingly recognized as a significant cause of traveler’s diarrhea. It is known for its “stacked brick” adherence pattern on epithelial cells and causes a persistent, watery diarrhea.
- The most important component in the treatment of V. cholerae infection is:
A. oral tetracycline and doxycycline
B. Anti-diarrheal medications
C. Correcting dehydration and salt depletion with water and electrolyte replenishment
D. NOTA
C. Correcting dehydration and salt depletion with water and electrolyte replenishment
Cholera can cause severe, life-threatening dehydration due to rapid loss of fluids and electrolytes from diarrhea. The primary treatment is rapid rehydration, either orally (Oral Rehydration Salts - ORS) or intravenously in severe cases.
- The Gram (-) enteric bacteria ferment lactose rapidly, is motile with flat, non- viscous colonies, which produce a metallic sheen in differential media.
A. E.coli
B. Shigella dysenteriae
C. Salmonella typhi
D. Proteus vulgaris
A. E.coli
E. coli is known for rapidly fermenting lactose, being motile, and producing distinctive metallic sheen colonies on Eosin Methylene Blue (EMB) agar, a differential medium.
- Blood culture results in this clinical disease induced by Salmonella will be positive frist to second weeks of infection.
A. Enteric fever
B. Enterocolitis
C. None of the choice are correct
D. Septicemia
A. Enteric fever
- These bacteria are gram-negative rods with comma, S, or “gull wing” shapes, motile with a single polar and do not form spores.
A. Campylobacter
B. Clostridium difficile
C. Bacillus cereus
D. Vibrio cholerae
A. Campylobacter
- What schedule to give tetanus toxoid will you recommend after a booster injection of tetanus toxoid is given upon school entry?
a. 5 years apart
b. 7 years apart
c. 10 years apart
d. 15 years apart
c. 10 years apart
- What part of the Haemophilus influenzae is the basis for typing?
a. Flagella
b. Plasmid
c. Nuclear membrane
d. Capsule
d. Capsule
- Tetanus is a totally preventable disease. What comprises the initial course of immunization?
a. 2 injections of tetanus toxoid
b. 3 injections of tetanus toxoid
c. 4 injections of tetanus toxoid
d. 5 injections of tetanus toxoid
b. 3 injections of tetanus toxoid
- What age is the DTap vaccine NOT recommended
a. 6 months old
b. 7 months old
c. 18 months old
d. 2 months old
b. 7 months old
- A 20/M came from rural Europe complained of high fever and body aches, swollen glands, and difficulty with swallowing which was present for weeks, the most likely organism that you’ll consider is?
a. Brucella sp
b. Pasteurulla multocida
c. Yersinia pestis
d. Francisella tularensis
c. Yersinia pestis
- What drug can be given as prophylaxis for non-immunized children exposed to a Pertussis case?
a. Rifampin
b. Cotrimoxazole
c. Clindamycin
d. Erythromycin
d. Erythromycin
- A person was exposed to an anthrax case, how long will the anthrax disease manifest?
a. 2 weeks
b. 8 weeks
с. 4 weeks
d. 6 weeks
a. 2 weeks
- Which organism causes highly commmunicable form of conjunctivitis and brazillian purpuric fever
a. H. aegypticus
b. H. ducreyi
c. H. aprophilus
d. H. haemolyticus
a. H. aegypticus
- What is the CBC picture of a Pertussis case?
a. Leukopenia with absolute lymphocytosis
b. Leukocytosis with segmenter predominance
c. Leucopenia with segmenter predominance
d. Leukocytosis with absolute lymphocytosis
d. Leukocytosis with absolute lymphocytosis
- Until what age is the infant protected from Haemophilus infection by antibodies from the mother?
a. under 3 months
b. under 5 months
c. under 7 months
d. under 9 months
a. under 3 months
- Which of the following statements is false regarding Brucella spp?
a. gram-positive and encapsulated coccobacilli
b. causes undulant fever
c. infected phagocytes carry brucella to the spleen
d. resistant to intracellular killing
a. gram-positive and encapsulated coccobacilli
- Which stage of Pertussis presents with nasal congestion, conjunctival suffusion and tearing?
a. Convalescent stage
b. Catarrhal stage
c. Prodromal stage
d. Paroxysmal stage
b. Catarrhal stage
- What age is the DTap vaccine NOT recommended?
a. 2 months old
b. 7 months old
c. 6 months old
d. 18 months old
b. 7 months old
- What organism can have human to human transmission?
a. Yersinia pestis
b. Pasteurella multocida
c. Brucella spp.
d. Francisella tularensis
a. Yersinia pestis
- A baby presented with severe paroxysmal coughing and cyanosis. What antibiotics will be suited for this condition?
a. Cephalosporins
b. Coamoxiclav
c. Penicillin
d. Macrolides
d. Macrolides
- Tetanus is a totally preventable disease. What comprises the initial course of immunization?
a. 4 injections of tetanus toxoid
b. 3 injections of tetanus toxoid
c. 5 injections of tetanus toxoid
d. 2 injections of tetanus toxoid
b. 3 injections of tetanus toxoid
- Botulism causes intoxication resulting from the ingestion of food in which has grown and produced toxins. When do Symptoms usually occur?
a. 18-24 hours after ingestion of the toxic food
b. 1-2 weeks
c. 4-5 days up to as many weeks
d. 20 minutes after consumption.
a. 18-24 hours after ingestion of the toxic food
- Which of the following food items is most frequently associated with infant botulism?
a. Corn syrup
b. Canned infant formula
c. Liquid multivitamins
d. Honey
d. Honey
- Which of the following produces paralysis due to an intoxication of types A or B toxin resulting from food ingestion with growth?
a. Clostridium tetani
b. Clostridium difficile
c. Clostridium botulinum
d. Clostridium perfringens
c. Clostridium botulinum
- What causes toxic shock syndrome?
a. Clostridium sordellii
b. Clostridium difficile
c. Clostridium septicum
d. Clostridium perfringens
a. Clostridium sordellii
- The spores germinate in the tissue at the site of entry, and growth of the vegetative organisms results in formation of a gelatinous edema and congestion
a. Bacillus anthracis
b. Bacillus cereus
c. Clostridium perfringens
d. Clostridium difficile
a. Bacillus anthracis
- What causes Pseudomembranous enterocolitis?
a. Clostridium difficile
b. Clostridium botulinum
c. Clostridium perfringens
d. Clostridium tetanus
a. Clostridium difficile
- Selective culture media for P. aeruginosa
a. Thayer martin agar
b. Blood agar
c. Colistin-containing agar
d. Brucella agar
b. Blood agar
- Enzyme Reaction caused by Bacteroides Fragilis
a. Ureases negative
b. Indole positive
c. Lipase Positive
d. beta-lactamase positive
d. beta-lactamase positive
- What management should you give in patients who develop symptoms of tetanus?
a. analgesics
b. 10,000 units antitoxin
c. 100 units antitoxin
d. assisted ventilation
b. 10,000 units antitoxin
- Enzyme reaction caused by Fusobacterium nucleatum
a. Beta lactamase positive
b. Lipase positive
c. Indole positve
d. Urease negative
a. Beta lactamases positive
- Enzyme reaction caused by Fusobacterium necrophorum
a. Lipase negative
b. Indole negative
c. Nitrate negative
d. Indole positive
d. Indole positive
- Enzyme reaction caused by achromobacter
a. Indole positive
b. Lipase positive
c. Urease negative
d. Beta lactamase positive
c. Urease negative
- Specific characteristic of Burkholderia mallei
a. Brown black halo
b. Disease of horses
c. pleomorphic
d. Gliding motility
b. Disease of horses
- Heparin is one of the tx for Lemierres disease
a. true
b. false
a. true
- An inanimate object that is capable of infecting and provide adequate environment for microorganism to multiply and reproduce.
a. Host
b. Reservoir host
c. Definitive host
d. Fomite
d. Fomite
- Catalase positive -
a. F. nucleatum
b. F. necrophorum
c. Both
d. None
a. F. nucleatum
- Indole positive -
a. F. nucleatum
b. F. necrophorum
c. Both
d. None
b. F. necrophorum
- Bile acid positive -
a. F. nucleatum
b. F. necrophorum
c. Both
d. None
a. F. nucleatum
- Nitrate positive -
a. F. nucleatum
b. F. necrophorum
c. Both
d. None
a. F. nucleatum
- Sensitive to Kanamycin -
a. F. nucleatum
b. F. necrophorum
c. Both
d. None
c. Both
- Nitrate negative
a. Fusobacterium nucleatum
b. Fusobacterium necrophorum
c. Both
d. Neither
b. Fusobacterium necrophorum
Positive: Indole/Lipase