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MODX > LAB2 - WORKFLOW AND EQUIPMENT > Flashcards

LAB2 - WORKFLOW AND EQUIPMENT Flashcards

1
Q

Unwanted Nucleic Acid into the specimen

A

CONTAMINATION

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2
Q

Repeated Amplification of the same target sequence leads to the accumulation of _____
(10^9) in the laboratory environment

A

Amplification products

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3
Q

Amplification products in PCR is called as

A

amplicons - 10^9

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4
Q

Types of Contamination

A

Contamination to the personnel
Contamination to the product
Contamination to the environment

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5
Q

sources of contamination

A

Cross contamination
Amplification product contamination
Laboratory surfaces
Ventilation ducts
Reagent/supplies
Hair, skin, saliva, and lab clothes of personnel

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6
Q

We process ________ samples each day
especially during this pandemic

A

500-1000

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7
Q

___ means to multiply copy of identical
nucleic acids that is used for diagnosis

A

Amplification

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8
Q

do we have amplicons in pre-analytical phase?

A

naur

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9
Q

COVID-19 testing shall only be done in a laboratory.

A

DOH licensed COVID-19 testing

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10
Q

the agency that will give the license to operate

A

DOH

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11
Q

the agency that will give the necessary compliance and guidelines

A

RITM
HFSRB/CHDRLED.

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12
Q

CHDRLED means

A

Center For Health Development - Regulatory, Licensing, and Enforcement Division

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13
Q

HFSRB means

A

Health Facilities and Services Regulatory Bureau

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14
Q

The COVID-19 testing laboratory shall be a separate
unit, with its own designated working room

true or false

A

true

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15
Q

COVID-19 testing laboratories shall have a _____ (Standard Operating Procedures)

A

Manual of Operations

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16
Q

first section in laboratory workstations

A

specimen receiving area

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17
Q

2nd section in laboratory workstations

A

sample inspevction and lysis room

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18
Q

3rd section in laboratory workstations

A

sample extraction toom

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19
Q

4th section in laboratory workstations

A

reagent mix and prep room

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20
Q

5th section in laboratory workstations

A

template addition room

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21
Q

6th section in laboratory workstations

A

PCR room

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22
Q

7th section in laboratory workstations

A

analysis room

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23
Q

a section that will receive the sample

A

sample receiving data such as oral nasal/oro phareangeal swabs

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24
Q

all samples that would be received by the molecular biology is in __ package

A

triple package

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25
Q

criteria for the triple package

A

-covered in absorbent material
- with resealable plastic and hazard symbol
- the resealable plastic must be further placed in another resealable plastic with another hazard p[lus patient identifier

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26
Q

who are qualified being receptionist

A

-any who are graduated in health related course

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27
Q

they received samples and ensure that the samples are triple pack and in proper storage such as in ice

A

receptionisit

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28
Q

the section wherein we conduct viral inactivation, and labeling of sample

A

sample inspection and lysis room

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29
Q

how do we conduct inactivation in section 2?

A

chemical - acid, bases, and those found in VTM
physical - temperature (50C to 60C)

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30
Q

person assigned in section 2 - sample inspection and lysis room

A

laboratory technician

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31
Q

section for obtaining very pure form of nucleic acids

A

section3 - sample extraction room

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32
Q

a section where we use different chemicals to extract nucleic acid

A

section3 - extraction room

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33
Q

an example of chemical which we use in section 3 for nucleic acid extraction

A

ELUATE

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34
Q

in reagent mix and prep room personnel

A

medtects

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35
Q

Reagent used in RT-PCR is _____ which are mixed together

A

master mix

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36
Q

pre-pcr areas or pre analytical area

A
  1. Specimen receiving area
  2. Sample inspection & lysis room
  3. Sample extraction room
  4. Reagent mix preparation room
  5. Template additional room
37
Q

called as clean areas due to lack of presence of amplicons

A
  1. Specimen receiving area
  2. Sample inspection & lysis room
  3. Sample extraction room
  4. Reagent mix preparation room
  5. Template additional room
38
Q

analytical area

A

pcr room

39
Q

post analytical area

A

analysis room

40
Q

LABORATORY WORKSTATIONS MINIMUM PHYSICAL
SEPARATION (DOH)

A

AREA 1- specimen reception (sec 1)
AREA 2 - viral inactivation and nucleic acid extraction (section 2,3,5)
AREA 3 - reagent storage and handling (section 4)
AREA 4 - pcr (section 6)
AREA 5 - clerical activities (section 7)

41
Q

out of the area based on minimum physical separation that must be the cleanest

A

area 3 - section 4 - reagent storage and handling

42
Q

procedures done in section 1

A

(1) receives and sorts samples;
(2) inspects documents;
(3) coordinates with healthcare facilities;
(4) releases results;
(5) facilitates transfer of materials from one station to another;
(6) coordinates supplies with supervisor

43
Q

physical set up in section 1

A

(1) computer;
(2) barcode printer;
(3) pass box;
(4) donning/doffing area;
(5) sink

44
Q

ppe in section 1

A

scrub suits,
disposable head cap,
surgical mask,
nitrile gloves,
disposable lab gown,
scrub suit,
lab shoes or shoe cover

45
Q

section 2 procedures

A

P7a – inspects and labels with barcode samples inside the BSC;
P7b – aliquot samples into two tubes (biobanking and processing); inactivates sample in one tube inside the BSC;
P8 – assists & monitors P7a/7b

46
Q

set up of section 2

A

(1) Class II biosafety cabinets (BSC);
(2) heat block inside the hood;
(3) centrifuge
(4) vortex mixer inside the hood;
(5) pipettors & tips;
(6) lysis reagent;
(7) pass box;
(8) refrigerator & freezer;
(9) ultra low freezer;
(10) biohazard disposal & cleaning supplies & autoclave;
(11) labelling area with barcode printer;
(12) donning/doffing area;
(13) wash area

47
Q

ppe in section 2

A

scrub suits,
lab shoes or shoe cover,
lab coat,
disposable lab gown, double layer nitrile gloves, , disposable head cap

N95/PAPR,
face shield

48
Q

procedure in section 3

A

: P2 – extracts RNA inside the BSC;
P3 – assists & monitors P2

49
Q

set up in section 3

A

(1) class II BSC;
(2) heat block;
(3) centrifuge;
(4) vortex mixer inside the hood;
(5) pipettors & tips;
(6) extraction kits & ethanol;
(7) biohazard disposal & cleaning supplies;
(8) refrigerator & freeze

50
Q

ppe in section 3

A

scrub suits,
lab shoes or shoe cover,
lab coat,
, disposable
lab gown, double layer nitrile gloves, disposable head cap

surgical mask

51
Q

procedure in section 4

A

all performed inside the laminar flow hood;

(1) prepares mastermix ;
(2) dispenses mastermix to PCR plate or tubes;
(3) adds negative controls to reaction

52
Q

set up in section 4

A

: (1) at least laminar flow hood/PCR cabinet (with UV lamp);
(2) vortex mixer inside/outside the hood;
(3) mini-centrifuge inside/outside the
hood;
(4) pipettors & tips;
(5) PCR reagents;
(6) cold rack;
(7) refrigerator &
freezer;
(8) disposal & cleaning supplies

53
Q

ppe in section 4

A

: scrub suits, surgical mask, nitrile gloves, lab coat, scrub suit, lab shoes or shoe cover

54
Q

procedure in section 6 and 7

A

facilitates PCR run & result interpretation

55
Q

physical set up in section 6 and 7

A

: (1) real time PCR machine;
(2) computer with software;
(3) cold rack;
(4) PCR plate or tube centrifuge; (5) disposal and cleaning supplies

56
Q

ppe in section 6 and 7

A

: scrub suit, surgical mask, nitrile gloves, lab shoes or shoe cover

57
Q

lab equipment

biosafety cabinet class required

A

class II - 70% exhau
sted, 30% recirculated

58
Q

autoclaving steam, time, and pressure

A

15 psi
15 mns
121*C

59
Q

VTM done in processing is stored in

A

-70*C biobase freezer

60
Q

biobanking stores in what freezer

A

-70*C biobase freezer

61
Q

frozen bacterial culture

A

-70*C freezer

62
Q

stores, annotate biological entities

A

biobanking

63
Q

0.2 ml to 0.5 ml of microcentrifuge tubes is for

A

pcr

64
Q

1.5 ml tube is for

A

extraction of nucleic acids

65
Q

for mixing of agarose gel

A

Spatula

66
Q

solution/components of VTM

A

hank’s balance salt solution
fetal bovine serum
amphotericin B 400 ug/ml
gentamicin sulfate 80 mg/2ml
sterile water for injection

67
Q

Hanks;
Balanced Salt
Solution components

A

calcium, magnesium,
and phenol red,
sterile

68
Q

Hanks;
Balanced Salt
Solution indicator

A

phenol red

69
Q

used to ensure enought nutrient for VTM

A

fetal bovine serum

70
Q

antibiotic that inhibits the gowth of fungi in VTM

A

amphotericin B

71
Q

antibiotic that inhibits the growth of bacteria in VTM

A

gentamicin sulfate

72
Q

buffer for electrophoresis

A

tris base

73
Q

act as enhancer of actviity of enzymes

A

mgcl2

74
Q

biological grade water

A

nuclease-free water

75
Q

dye for bands of electrophoresis

A

gel loading dye or buffer

76
Q

gel loading used that is NOT cancerous

A

GEL RED

77
Q

GEL RED replaced __

A

ethidium bromide

78
Q

component of PCR kit

A

dNTPs, MgCl2,
Taq polymerase, buffer

79
Q

disrupts protein

A

Proteinase K

80
Q

reagents stored in room temp

A

detergents
ethanol
buffer
concentrated solution
acids and bases

81
Q

4*C storage

A

bacterial cultures
buffers
dna
media
pcr products
serum

82
Q

-20*C storage

A

enzyme
RNA

83
Q

-70*C

A

frozen bacterial cultures
lipids

84
Q

Hazardous Chemicals: we must refer to ______ which describes the composition and properties,
toxicology, and instructions for handling, spill control, and
waste disposal.

A

MSDS (Materials Safety Data
Sheet)

85
Q

brand for Extraction only

A

(KingFisher)

86
Q

brand for Extraction &
dispensing

A

(Sansure Natch CS)

87
Q

brand for Extraction,
dispensing, and qPCR

A

bd max

88
Q

the section that is most prominnet in acquiring infection

A

section2

89
Q
A

MODX (9 decks)

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