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MODX > LAB- SPECIMEN COLLECTION > Flashcards

LAB- SPECIMEN COLLECTION Flashcards

1
Q

Congress passed the _____ in 1988 to establish quality testing standards to ensure consistent patient test results.

A

Clinical Laboratory Improvement Amendments (CLIA)

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2
Q
  • CLIA specifies
A

quality standards for proficiency testing, patient test management
, quality control,
personnel qualifications,
and quality assurance for laboratories performing moderate- and/or high-complexity tests, including molecular testing

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3
Q

regulatory agency/ governing body for molbio

A

Clinical Laboratory Improvement Amendments (CLIA)

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4
Q

the success of a test procedure is affected by the _____

A

age of the specimen
type of the specimen
condition of the specimen

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5
Q

test we do usually required the following

patient consent forms,
ethnicity
photo identification of the individuals tested
patient label verification
transfusion history
pedigree may also be supplied with the test specimen

A

for molecular genetics
forensics
parentage testing

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6
Q

___ are required for cabinets, refrigerators, or freezers that contain potentially hazardous reagents or patient specimens..

A

Biohazard stickers

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7
Q

Molecular genetic tests may require dedicated specimens not shared with other tests

true or false

A

true

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8
Q

can Hemoglobin inhibits enzyme activity

A

yes

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9
Q

since hemoglobin can inhibits enzyme activity

Specimens received in the laboratory should therefore be inspected for visual signs of __

A

hemolysis

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10
Q
A
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11
Q

if the WBC lysis occured, what would be the effect to rna or dna yield

A

reduced

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12
Q

effect of white blood cell lysis

A

false negative result in qualitative testing
inaccurate measurement in quantitative analyses

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13
Q

2 component to add to prevent interfering substances

A

resin and buffer

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14
Q

type of dna we can found in fixed tissues

A

short dna

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15
Q

for southern blot or long range PCR is from what tissue

A

fresh tissues

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16
Q

short dna from fixed tissue is only done with what test

A

for real time PCR

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17
Q

best method to freeze or preserve tissue without fixation

A

liquid nitrogen - snap freezing

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18
Q

Fixed, paraffin-embedded tissues generally yield ____-quality DNA and RNA

A

lower

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19
Q

PCR and reverse transcription PCR (RT-PCR) amplification are routinely performed on ___ tissue samples.

A

paraffin-embedded

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20
Q

the only antocoagulant we can use for nucleic acid testing of blood

A

tripotassium edta and acid citrate dextrose

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21
Q

why we must not use heparin

A

inhibits pcr enzymes - taq polymerase and RT
coextracted at DNA extraction

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22
Q

red top tube is for what nucleic acid testing

A

chemistry, serum, viral antibody studies

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23
Q

green top tube is for what nucleic acid testing

A

immunology, virology studies

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24
Q

what is the additive of green tube vacutainer

A

sodium heparin (freeze dried )

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25
Q

what is the additive if the brown top vacutainer

A

sodium heparin

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26
Q

brown top tube is for what nucleic acid testing

A

cytogenetic studies, molecular studies

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27
Q

lavender top tube is for what nucleic acid testing

A

virology, molecular biology studies

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28
Q

lavender top tube additive

A

tripotassium EDTA — 7.5 % - 15% solution

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29
Q

yellow top tube is for what nucleic acid testing

A

molecular biology studies - acid citrate dextrose

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30
Q

___ are designed to stabilize RNA.

A

Specialty collection tubes

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31
Q

Specialty collection tubes can contain

A

DEPC - diethyl pyrocarbonate
ethanol - precipitating nucleic acid

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32
Q

we must store the rna sample at _____ to stabilize RNA for several days

A

–70°C

33
Q

we can use An alternative to standard collection tubes is the use of impregnated paper matrices for nucleic acid preservation and storage.

true or false

A

true

34
Q

we can use An alternative to standard collection tubes is the use of ___for nucleic acid preservation and storage.

A

impregnated paper matrices

35
Q

recommended temperature for RNA storage

A

-20C and -70C

36
Q

is 22C and 25C temperature storage for RNA recommended

A

no po

37
Q

interview form which the symptoms of the patient, as well as the close contact from the past few days

A

COVID-19 Case Investigation Form (CIF) and RITM linelist

38
Q

nasopharyngeal swab we must used

A

Dacron/Rayon swab with pliable shaft - we can’t use calcium alginate swab as it inhibts PCR

39
Q

Dacron/Rayon swab is made up of ___

A

artificial cotton so it will not prevent the PCR

40
Q

Oropharyngeal swab,

A

Sterile Dacron/Rayon swab with plastic shaft

41
Q

Swabs should not be used

A

there is evidence of damage or contamination
the expiration date has passed
the swab package is damaged
there are other signs of deterioration

42
Q

During sampling, the swab shall only come in contact with the suspected infection

true or false

A

true

43
Q

the time of collection for swabbing

A

2-3 days - high IgM - acute infection

44
Q

Do not use excessive force when collecting swab samples from patients as this may result in accidental breakage of the swab shaft

true or false

A

true

45
Q

Swabs are for single use only

true or false

A

true

46
Q

site of swabbing

A

choana or posterior wall of nasopharynx

47
Q

travel distance of nasopharynx

A

5-6 cm or 2 inches

48
Q

site for oropharyngeal swab

A

palatine tonsils
posterior wall of oropharynx

49
Q

A. Specimens shall be collected within ___ days from onset of illness

A

10

50
Q

Only qualified and trained staff shall perform the procedures

true or false

A

true

51
Q

Do use wooden and cotton swabs

true or false

A

false

52
Q

.Check for integrity of the OPS/NPS swabs and do not use beyond expiration.

true or false

A

true

53
Q

Use only the approved kits for specimen collection.

A

oki

54
Q

specimen collection policies

A

correctly identify the patient and label the UTM tube prior to collection

label the tube with the patient’s full name, age, and date of collection. The information on the label must match the information on the CIF

strictly follow infection control guidelines prior to each procedure (protect yourself from the patient)

55
Q

Using the swab, visually measure from the base of the nostril towards the __

A

auditory pit

56
Q

With the patient seated, tilt the head slightly backwards. Insert the swab into the nostril
___ to the palate

A

parallel

57
Q

Insert the swab into the nasal cavity until ___

A

a slight resistance is met

58
Q

upon insertion of swab, we must __

A

Rotate the swab and apply a little force to take large quantities of mucosa

59
Q

do we Repeat in the other nostril using same swab upon nasopharyngeal swabbing?

A

ye

60
Q

we are placing the NPS immediately in the UTM tube to avoid ___

A

drying of the swab

61
Q

after creaking the end of the shaft that sticks out of the tube and close the tube tightly, we secure the cap with ___ to prevent leakage during transport

A

parafilm

62
Q

we transport the specimen to the laboratory after collection and immediately store inside what temperature

A

2-8*C

63
Q

if the site is far from the refrigerators, use ____ during transportation

A

thermo bag/box with 4-6 icepacks

64
Q

during oropharyngeal swabbing, we have the patient to say _____ to elevate the uvula

A

ahh

65
Q

we use a ___ motion to swab the posterior pharyngeal wall and tonsillar fossa

A

sweeping

66
Q

we must avoid ___ in OPS

A

soft palate
tongue

67
Q

after collection through OPS we immediately put it in ___

A

the same UTM where the NPS is stored

68
Q

best practices in specimen collection

A

ask patient to remove extra secretions before procedure

ask patient to close his or eyes

visually estimate the depth of insertion

label the tube first prior to collection

remember your safety first and foremost

69
Q

we store the VTM if unused ___

A

room temp - no light

70
Q

we store the VTM if used ___

A

2-8*C

71
Q

A. Package, Shipped and Transport: According to the current edition of the ___ Dangerous Goods Regulation

A

International Air Transport Association (IATA)

72
Q

Extracted nucleic acid should be stored at __

A

-70°C or Lower

73
Q

If a specimen is frozen at -70°C, ship on ___

A

dry ice

74
Q

st6orage: Store specimens at __ up to 72 hrs. and ship on ice pack

A

2-8°C

75
Q

gold standard sample in the detection of covid 19

A

nasopharyngeal swab

76
Q

most sensitive in covid testing

A

bronchial washing

77
Q

recommended when sending potential 2019-nCoV specimens

A

Follow shipping regulations for UN 3373 Biological Substance, Category B

78
Q
A

MODX (17 decks)

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