LAB- SPECIMEN COLLECTION

Question 1

Congress passed the _____ in 1988 to establish quality testing standards to ensure consistent patient test results.

Answer 1

Clinical Laboratory Improvement Amendments (CLIA)

Question 2

• CLIA specifies

Answer 2

quality standards for proficiency testing, patient test management
, quality control,
personnel qualifications,
and quality assurance for laboratories performing moderate- and/or high-complexity tests, including molecular testing

Question 3

regulatory agency/ governing body for molbio

Answer 3

Clinical Laboratory Improvement Amendments (CLIA)

Question 4

the success of a test procedure is affected by the _____

Answer 4

age of the specimen
type of the specimen
condition of the specimen

Question 5

test we do usually required the following

patient consent forms,
ethnicity
photo identification of the individuals tested
patient label verification
transfusion history
pedigree may also be supplied with the test specimen

Answer 5

for molecular genetics
forensics
parentage testing

Question 6

___ are required for cabinets, refrigerators, or freezers that contain potentially hazardous reagents or patient specimens..

Answer 6

Biohazard stickers

Question 7

Molecular genetic tests may require dedicated specimens not shared with other tests

true or false

Answer 7

true

Question 8

can Hemoglobin inhibits enzyme activity

Answer 8

yes

Question 9

since hemoglobin can inhibits enzyme activity

Specimens received in the laboratory should therefore be inspected for visual signs of __

Answer 9

hemolysis

Question 11

if the WBC lysis occured, what would be the effect to rna or dna yield

Answer 11

reduced

Question 12

effect of white blood cell lysis

Answer 12

false negative result in qualitative testing
inaccurate measurement in quantitative analyses

Question 13

2 component to add to prevent interfering substances

Answer 13

resin and buffer

Question 14

type of dna we can found in fixed tissues

Answer 14

short dna

Question 15

for southern blot or long range PCR is from what tissue

Answer 15

fresh tissues

Question 16

short dna from fixed tissue is only done with what test

Answer 16

for real time PCR

Question 17

best method to freeze or preserve tissue without fixation

Answer 17

liquid nitrogen - snap freezing

Question 18

Fixed, paraffin-embedded tissues generally yield ____-quality DNA and RNA

Answer 18

lower

Question 19

PCR and reverse transcription PCR (RT-PCR) amplification are routinely performed on ___ tissue samples.

Answer 19

paraffin-embedded

Question 20

the only antocoagulant we can use for nucleic acid testing of blood

Answer 20

tripotassium edta and acid citrate dextrose

Question 21

why we must not use heparin

Answer 21

inhibits pcr enzymes - taq polymerase and RT
coextracted at DNA extraction

Question 22

red top tube is for what nucleic acid testing

Answer 22

chemistry, serum, viral antibody studies

Question 23

green top tube is for what nucleic acid testing

Answer 23

immunology, virology studies

Question 24

what is the additive of green tube vacutainer

Answer 24

sodium heparin (freeze dried )

Question 25

what is the additive if the brown top vacutainer

Answer 25

sodium heparin

Question 26

brown top tube is for what nucleic acid testing

Answer 26

cytogenetic studies, molecular studies

Question 27

lavender top tube is for what nucleic acid testing

Answer 27

virology, molecular biology studies

Question 28

lavender top tube additive

Answer 28

tripotassium EDTA --- 7.5 % - 15% solution

Question 29

yellow top tube is for what nucleic acid testing

Answer 29

molecular biology studies - acid citrate dextrose

Question 30

___ are designed to stabilize RNA.

Answer 30

Specialty collection tubes

Question 31

Specialty collection tubes can contain

Answer 31

DEPC - diethyl pyrocarbonate ethanol - precipitating nucleic acid

Question 32

we must store the rna sample at _____ to stabilize RNA for several days

Answer 32

–70°C

Question 33

we can use An alternative to standard collection tubes is the use of impregnated paper matrices for nucleic acid preservation and storage. true or false

Answer 33

true

Question 34

we can use An alternative to standard collection tubes is the use of ___for nucleic acid preservation and storage.

Answer 34

impregnated paper matrices

Question 35

recommended temperature for RNA storage

Answer 35

-20*C and -70*C

Question 36

is 22*C and 25*C temperature storage for RNA recommended

Answer 36

no po

Question 37

interview form which the symptoms of the patient, as well as the close contact from the past few days

Answer 37

COVID-19 Case Investigation Form (CIF) and RITM linelist

Question 38

nasopharyngeal swab we must used

Answer 38

Dacron/Rayon swab with pliable shaft - we can't use calcium alginate swab as it inhibts PCR

Question 39

Dacron/Rayon swab is made up of ___

Answer 39

artificial cotton so it will not prevent the PCR

Question 40

Oropharyngeal swab,

Answer 40

Sterile Dacron/Rayon swab with plastic shaft

Question 41

Swabs should not be used

Answer 41

there is evidence of damage or contamination the expiration date has passed the swab package is damaged there are other signs of deterioration

Question 42

During sampling, the swab shall only come in contact with the suspected infection true or false

Answer 42

true

Question 43

the time of collection for swabbing

Answer 43

2-3 days - high IgM - acute infection

Question 44

Do not use excessive force when collecting swab samples from patients as this may result in accidental breakage of the swab shaft true or false

Answer 44

true

Question 45

Swabs are for single use only true or false

Answer 45

true

Question 46

site of swabbing

Answer 46

choana or posterior wall of nasopharynx

Question 47

travel distance of nasopharynx

Answer 47

5-6 cm or 2 inches

Question 48

site for oropharyngeal swab

Answer 48

palatine tonsils posterior wall of oropharynx

Question 49

A. Specimens shall be collected within ___ days from onset of illness

Answer 49

10

Question 50

Only qualified and trained staff shall perform the procedures true or false

Answer 50

true

Question 51

Do use wooden and cotton swabs true or false

Answer 51

false

Question 52

.Check for integrity of the OPS/NPS swabs and do not use beyond expiration. true or false

Answer 52

true

Question 53

Use only the approved kits for specimen collection.

Answer 53

oki

Question 54

specimen collection policies

Answer 54

correctly identify the patient and label the UTM tube prior to collection label the tube with the patient's full name, age, and date of collection. The information on the label must match the information on the CIF strictly follow infection control guidelines prior to each procedure (protect yourself from the patient)

Question 55

Using the swab, visually measure from the base of the nostril towards the __

Answer 55

auditory pit

Question 56

With the patient seated, tilt the head slightly backwards. Insert the swab into the nostril ___ to the palate

Answer 56

parallel

Question 57

Insert the swab into the nasal cavity until ___

Answer 57

a slight resistance is met

Question 58

upon insertion of swab, we must __

Answer 58

Rotate the swab and apply a little force to take large quantities of mucosa

Question 59

do we Repeat in the other nostril using same swab upon nasopharyngeal swabbing?

Answer 59

ye

Question 60

we are placing the NPS immediately in the UTM tube to avoid ___

Answer 60

drying of the swab

Question 61

after creaking the end of the shaft that sticks out of the tube and close the tube tightly, we secure the cap with ___ to prevent leakage during transport

Answer 61

parafilm

Question 62

we transport the specimen to the laboratory after collection and immediately store inside what temperature

Answer 62

2-8*C

Question 63

if the site is far from the refrigerators, use ____ during transportation

Answer 63

thermo bag/box with 4-6 icepacks

Question 64

during oropharyngeal swabbing, we have the patient to say _____ to elevate the uvula

Answer 64

ahh

Question 65

we use a ___ motion to swab the posterior pharyngeal wall and tonsillar fossa

Answer 65

sweeping

Question 66

we must avoid ___ in OPS

Answer 66

soft palate tongue

Question 67

after collection through OPS we immediately put it in ___

Answer 67

the same UTM where the NPS is stored

Question 68

best practices in specimen collection

Answer 68

ask patient to remove extra secretions before procedure ask patient to close his or eyes visually estimate the depth of insertion label the tube first prior to collection remember your safety first and foremost

Question 69

we store the VTM if unused ___

Answer 69

room temp - no light

Question 70

we store the VTM if used ___

Answer 70

2-8*C

Question 71

A. Package, Shipped and Transport: According to the current edition of the ___ Dangerous Goods Regulation

Answer 71

International Air Transport Association (IATA)

Question 72

Extracted nucleic acid should be stored at __

Answer 72

-70°C or Lower

Question 73

If a specimen is frozen at -70°C, ship on ___

Answer 73

dry ice

Question 74

st6orage: Store specimens at __ up to 72 hrs. and ship on ice pack

Answer 74

2-8°C

Question 75

gold standard sample in the detection of covid 19

Answer 75

nasopharyngeal swab

Question 76

most sensitive in covid testing

Answer 76

bronchial washing

Question 77

recommended when sending potential 2019-nCoV specimens

Answer 77

Follow shipping regulations for UN 3373 Biological Substance, Category B