GNA EDITING Flashcards

1
Q

a microbial immune system that has been repurposed as a genome editing tool

A

CRISPR

clustered regularly interspaced short palindromic repeats

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2
Q

the process by which a molecule is introduced to a particular cell or organism

A

delivery

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3
Q

a nucleic acid based binding domain used in CRISPR genome editing

A

guide

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4
Q

customized binding domain of gRNA

A

guide

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5
Q

a mechanism in cells that allows double-stranded breaks in DNA to be repaired without the availability of a homologous template

A

non homologous end joining

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6
Q

nuclease protein that can cleave nucleic acids

A

payload

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7
Q

restriction enzymes that can be engineered to cut specific sequences of DNA in the genome

A

TALENs

transcription activator like effector nucleases

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8
Q

artificial restriction enzymes that can be engineered to cut specific sequences of genomic DNA

A

ZFN

zinc finger nucleases

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9
Q

Umbrella term for any type of manipulation with an organism’s DNA

A

GENETIC ENGINEERING AND GENE EDITING

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10
Q

Approaches OF GENETIC ENGINEERING AND EDITING

A

o Recombination (Mice,Yeast)
o Transposable Elements an Bacterial insertion of DNA
o Transfection of gene constructs into cells
o Genome Editing

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11
Q

steps in genetic engineering

A
  1. isolate the gene
  2. insert the gene in a host using a vector (plasmid vector)
  3. produce many copies of the host
  4. separate and purify the products of the gene
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12
Q

steps in genetic engineering in bacteria

A

grow the microorganism
extract the DNA of bacteria
get the target DNA
use the restriction enzymes to get the target DNA
insert the DNA fragments in plasmid cloning vector then transfer to another organism of interest

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13
Q

example of genetic engineering to bacteria

A

vibrio to e.coli

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14
Q

group of technologies that gives scientist the ability to change an organism’s DNA

A

gene editing

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15
Q

add, remove, replace, or modify multiple genes with a high degree of precision

A

gene editing

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16
Q

artifical restriction enzymes that can be engineered to cut specific sequences of genomic DNA

A

zinc finger nucleases (ZFNs)

17
Q

series of DNA binding domains assembled to recognized a specific sequence

A

transcription activator-like effector nucleases (TALENs)

18
Q

guide RNA directs Cas9 protein to target site

A

CRISPR system

19
Q

it creates an RNA guide

A

CRISPR system

20
Q

it will cut a particular domain of DNA that has zinc finger

A

zinc finger nucleases

21
Q

one of the application of ZFN is engineering of the genes __

A

HIV

22
Q

genetic modification of the receptor of HIV

A

CXCR5

23
Q

it edits the receptor of HIV to search for the possible treatments of HIV

A

ZFNs

24
Q

mostly used in diagnosing hematological diseases

editing the sickle cell anemia

A

TALENS - transcription activator-like effector nucleases

25
Q

rAAV means

A

recombinant adeno associated virus

26
Q

uses a virus as a vector to carry DNA into a cell

A

recombinant adeno associated virus

27
Q

incorporation of modified DNA into the genome

A

homologous recombination

28
Q

highly specific, efficient, and well suited for high throughout gene editing for diverse set of cell types

A

CRISPR SYSTEM

29
Q

CRISPR SYSTEM perks

A

faster
cheaper
accurate
efficient than other methods

30
Q
A