GNA EDITING Flashcards
a microbial immune system that has been repurposed as a genome editing tool
CRISPR
clustered regularly interspaced short palindromic repeats
the process by which a molecule is introduced to a particular cell or organism
delivery
a nucleic acid based binding domain used in CRISPR genome editing
guide
customized binding domain of gRNA
guide
a mechanism in cells that allows double-stranded breaks in DNA to be repaired without the availability of a homologous template
non homologous end joining
nuclease protein that can cleave nucleic acids
payload
restriction enzymes that can be engineered to cut specific sequences of DNA in the genome
TALENs
transcription activator like effector nucleases
artificial restriction enzymes that can be engineered to cut specific sequences of genomic DNA
ZFN
zinc finger nucleases
Umbrella term for any type of manipulation with an organism’s DNA
GENETIC ENGINEERING AND GENE EDITING
Approaches OF GENETIC ENGINEERING AND EDITING
o Recombination (Mice,Yeast)
o Transposable Elements an Bacterial insertion of DNA
o Transfection of gene constructs into cells
o Genome Editing
steps in genetic engineering
- isolate the gene
- insert the gene in a host using a vector (plasmid vector)
- produce many copies of the host
- separate and purify the products of the gene
steps in genetic engineering in bacteria
grow the microorganism
extract the DNA of bacteria
get the target DNA
use the restriction enzymes to get the target DNA
insert the DNA fragments in plasmid cloning vector then transfer to another organism of interest
example of genetic engineering to bacteria
vibrio to e.coli
group of technologies that gives scientist the ability to change an organism’s DNA
gene editing
add, remove, replace, or modify multiple genes with a high degree of precision
gene editing
artifical restriction enzymes that can be engineered to cut specific sequences of genomic DNA
zinc finger nucleases (ZFNs)
series of DNA binding domains assembled to recognized a specific sequence
transcription activator-like effector nucleases (TALENs)
guide RNA directs Cas9 protein to target site
CRISPR system
it creates an RNA guide
CRISPR system
it will cut a particular domain of DNA that has zinc finger
zinc finger nucleases
one of the application of ZFN is engineering of the genes __
HIV
genetic modification of the receptor of HIV
CXCR5
it edits the receptor of HIV to search for the possible treatments of HIV
ZFNs
mostly used in diagnosing hematological diseases
editing the sickle cell anemia
TALENS - transcription activator-like effector nucleases
