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Micro > Lab test 1 > Flashcards

Lab test 1 Flashcards

0
Q

Magnify the specimen-rotate

A

Objective lenses

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1
Q

Eye piece

A

Ocular lense

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2
Q

Attaches objective lenses to microscope

A

Revolving nosepiece

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3
Q

Where the slide is placed

A

Mechanical stage

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4
Q

Adjust light

A

Iris diaphragm lever

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5
Q

What you hold microscope with

A

By arm

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6
Q

Larger knob

A

Coarse adjustment

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7
Q

Small adjustment

A

Fine adjustment

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8
Q

Moves stage back and forth

A

Mechanical stage control

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9
Q

The enlargement of te image by use of the various objective and the ocular

A

Magnification

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10
Q

Ability to distinguish between two adjacent points

A

Resolution

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11
Q

The diameter of the smallest objective visible

A

Resolving power

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12
Q

Sphere or round

A

Coccus

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13
Q

Rods

A

Bacillus

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14
Q

Helixes or spiral

A

Spirillum

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15
Q

Total magnification=

A

Objective power x 10

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16
Q

Growth of cells of one specific microorganism

A

Pure culture

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17
Q

A medium may be a

A

Liquid or soli

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18
Q

Solid medium will have

A

Agar

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19
Q

Must be heated to boiling to dissolve and will solidify at 40C

A

Agar

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20
Q

Infection origination in a hospital

A

Nosocomial Infection

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21
Q

_____ a slide of bacteria improves the contrast between the cells and the background

A

Staining

22
Q

Disadvantage of staining?

A

Drying and fixation of the cells onto a slide introduces some shrinkage and distortion to cell

23
Q

Will kill the cells and also ensure that they will stay attached tithe glass surface during staining

A

Fixation

24
Q

Distinguish between different organisms on the basis of staining reactions

A

Differential stains

25
Q

Most useful differential stain in microbiology

A

Gram stain

26
Q

Differentiates cells on te basis of cell wall composition

A

Gram stain

27
Q

No lipid in their cell wall
Decolonize with ethanol or acetone
Retain crystal violet
Purple

A

Gram positive

28
Q

Decolonize and accept the counterstain

Appear pink

A

Gram negative

29
Q

What type of bacteria do you do acid stain for?

A

Mycobacterium
Nocardia
Staphylococcus

30
Q

Acid dye that stains background

A

Negative stain

31
Q

Used a modified negative stain with both the background and cells being stained

A

Capsule stain

32
Q

_____ remains unstained, giving the cells a halo appearance

A

Capsule stain

33
Q

Uses heat to penetrate the spore with the dye

A

Endospore stain

34
Q

All over flagella

A

Peritrichous

35
Q

Multiple flagella on one end

A

Polar lophotrichous

36
Q

1 flagella on one end

A

Polar monotrichous

37
Q

Flagella on both ends

A

Polar Amphitrichous

38
Q

Uses a needle

A

Motility test

39
Q

Indicated by growth away from the line of inoculation

A

Motility test

40
Q

Steps of motility test

A

Heat needle
Obtain organism
Stab straight down 1 time

41
Q

Enzymes that function outside the cell

A

Exoenzymes

42
Q

Enzymes that function inside the cell

A

Endoenzyme

43
Q

Collects gas produced during fermentation

A

Durham tube

44
Q

Used in the fermentation of lactose

A

Durham tube

45
Q

Anaerobic process

A

Fermentation of lactose

46
Q

Test tube that has a second inverted tube inside used to capture fermented gas

A

Durham tube

47
Q

One or more components that inhibit most microorganisms and allows a select group to grow

A

Selective media

48
Q

Contain indicators that allow the distinguishing of different organisms on the basis of their appearance on the media or the color of the media around the growth

A

Differential media

49
Q

Mannitol salt agar

A

Selective

Differential

50
Q

MacConkey’s Agar

A

Selective

Differential

51
Q

Blood agar

A

Differential

52
Q

Lowenstein-Jensen

A

Selective

53
Q

Sabouraud’s dextrose agar

A

Selective

Micro (10 decks)

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