Chapter 5- Microbe Metabolism Flashcards
Metabolism=
Catabolism+anabolism
The sum of the chemical elections in an organism
Metabolism
Provides energy and building blocks for anabolism
Catabolism
Uses energy and building looks to bills large molecules
Anabolism
The most common carb
Glucose
Catabolism releases energy by
Oxidation of molecules
Anabolism uses energy to
Synthesize macromolecules that make up the cell
Sequence of enzymatically catalyzed chemical reactions determine by enzymes
Metabolic pathway
What are enzymes
Proteins that do work
Enzymes lower the energy of
Activation
Specific for a chemical reaction but not used up in that reaction
Biological catalysts
Apoenzyme
Protein
Cofactors
Non protein component (vitamin, mineral, metal ion)
Holoenzyme=
Apoenzyme+ cofactors
Substrate=
Apoenzyme+coenzyme
Where the substrate binds to the enzyme
Active site
Enzymes end in
Ase
Enzyme used to make ATP
ATPase
ATP
- stores energy in phosphate bonds
- energy stored in phosphate bonds
- nearly universal energy molecule for living organisms
Factors influencing enzyme activity
Temp, pH, substrate concentration, inhibitors
Temp and pH _____ proteins
Denature-when heated=breaks down
Inhibitor that binds to active site
Competitive inhibition
Inhibitor that binds to a site other than active site
Non competitive inhibition
Site where the non competitive inhibition binds
Allosteric site
Enzymatic pathways require control so cells may regulate enzyme activity. Control is achieved via
Feedback inhibition
Removal of electrons
Oxidation
Gain of electrons
Reduction
An oxidation reaction paired with a reduction reaction
Redox reaction
The breakdown of carbohydrates to release energy
Carbohydrate catabolism
Breakdown of glucose from 6 carbon to 3 carbon
Glycolysis
Further breakdown or glycolysis product
Krebs
Oxidation reduction chain to produce ATP
Electron transport chain
True false: fermentation goes to Krebs
False
True false: respiration goes to Krebs
True
The oxidation of glucose to pyruvic acid produces ATP and NADH
Glycolysis
4 ATP produced, 2 ATP used, 2 net ATP are produced, 2 NADH produced, 2 pyruvic acid
Glycolysis
Oxidation of molecules liberates electrons for an electron transport chain
Cellular respiration
ATP is generated by oxidative phosphorylation
Cellular respiration
Oxidation of acetyl CoA produces NADH and FADH2
Krebs cycle
NADH and FADH2 deliver electrons and hydrogen ions to the ETC
Electron transport chain
Electrons are transferred between the chain molecules by a series of oxidation reduction steps
ETC
This cases hydrogen ions to move across a membrane and build up a high concentration on one side
ETC
Hydrogen ions move down their concentration gradient through ATP synthase, which drives synthesis of ATP when O2 is available
ETC
ETC site in eukaryotic cell
Inner mitochondrial membrane
ETC site in prokaryotic cell
Plasma membrane
The final electron acceptor in the electron transport chain is molecular O2
Aerobic respiration
The final electron acceptor in ETC is not O2
Anaerobic respiration
Why does anaerobic respiration yield less energy than aerobic respiration?
Because only part of the Krebs cycle operates under anaerobic conditions
Inorganic electron acceptor
No O2 require
Anaerobic respiration
Energy produced from a complete oxidation of one glucose using aerobic respiration
Carbohydrate catabolism
Any spoilage of food by microorganisms
Fermentation
Any process that produced alcoholic beverages or acidic dairy products
Fermentation
Any large scale microbial process occurring with or without air
Fermentation
Releases energy from oxidation of organic molecules
Does no require O2
Does not use Krebs or ETC
Fermentation.
Uses organic molecule as final electron acceptor
Fermentation
Conversion of light energy to chemical energy
Photosynthesis
Carbon fixation and light independent (dark) reaction
Synthesis
Fixing carbon into organic molecules
Synthesis
Calvin benson cycle
Light independent/ dark reaction