Lab Techniques Flashcards
Fill in the blanks: In gel electrophoresis, the anode has a _________ charge and the cathode has a _________ charge. This is an example of a(n) __________ cell.
Positive charge, negative charge.
This is an electrolytic cell, not a galvanic cell. Current is being applied to force molecules to move in the intended direction, usually towards the anode.
True or False: Gel electrophoresis uses the set up of a electrolytic cell
TRUE: This is an electrolytic cell, not a galvanic cell. Current is being applied to force molecules to move in the intended direction, usually towards the anode.
What is hybridization used for in PCR?
Hybridization is the process wherein single-stranded DNA or RNA forms base pairs with complementary DNA or RNA. This is used in PCR, or polymerase chain reaction, to anneal DNA strands to each other in order to artificially replicate them.
What are the three fundamental steps of blotting?
- Separate biomolecule by size using gel electrophoresis
- Transfer to a membrane
- Treat it with a detecting molecule conjugate probe
Which blot measures DNA?
Southern
Which blot measures RNA?
Northern
Which blot measures protein?
Western
How can you remember the blotting?
SNoW DRoP
What are DNA microarrays used to study?
Tens to thousands of gene expression profiles. Can compare the relative expression between two different cell types.
Describe the process of PCR
You first heat your genetic material to a toasty 95C so that it denatures, then primers will anneal to your target sequences once you’ve done cooled it to 55-65C, then you boil that bad boy back up to 72C and taq polymerase swoops in an then you start the whole shabang over
True or False: you can use gel electrophoresis to detect levels of gene expression
PCR cannot be used to quantitate gene expression - this must be done with qPCR/RT-PCR.
