Lab Review Flashcards

1
Q

what is the scientific method

A

a method of procedure that has characterized natural science since the 17th century, consisting in systematic observation, measurement, and experiment, and the formulation, testing, and modification of hypotheses.

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2
Q

steps of the scientific method

A
  1. Make an observation.
  2. Ask a question.
  3. Form a hypothesis, or testable explanation.
  4. Make a prediction based on the hypothesis.
  5. Test the prediction.
  6. Iterate: use the results to make new hypotheses or predictions.
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3
Q

what is a hypothesis

A

a supposition or proposed explanation made on the basis of limited evidence as a starting point for further investigation.

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4
Q

comparative vs controlled experiments

A

comparative = Involves collecting data on different populations/organisms under different conditions (ex. Times of year, Locations), to make a comparison.

controlled = an experiment in which all the variable factors in an experimental group and a comparison control group are kept the same except for one variable factor in the experimental group that is changed or altered

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5
Q

independent vs dependent vs standardized variables

A

independent = a variable (often denoted by x ) whose variation does not depend on that of another.

dependent = a variable (often denoted by y ) whose value depends on that of another.

standardized = a variable that has been rescaled to have a mean of zero and a standard deviation of one

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6
Q

conversion of m and nm

A

1m=100cm 10^2
1000mm 10^3
1000000um 10^6
1000000000nm 10^9

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7
Q

what are are the four major macromolecules

A

lipids, carbohydrates, proteins, and nucleic acids

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8
Q

what is osmosis

A

a process by which molecules of a solvent tend to pass through a semipermeable membrane from a less concentrated solution into a more concentrated one

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9
Q

hypertonic means

A

a solution has a higher concentration of solute than is found in the cells and the water leaves the cell

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10
Q

hypotonic means

A

a solution has a lower solute concentration than the cells and the cells take up water

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11
Q

isotonic means

A

an extracellular solution which contains the same concentration as the solute

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12
Q

what happens to a cell in a hypertonic solution

A

in plant and animal the cells shrink

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13
Q

what happens to a cell in a hypotonic solution

A

in plant they swell and increase turgor pressure and in animal they burst

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14
Q

what happens to a cell in a isotonic solution

A

nothing

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15
Q

what is ATP

A

the immediate energy source for most of a cells activities

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16
Q

what is potential energy in a cell

A

the energy stored in bonds of the. molecule

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17
Q

number of carbons in glucose and fructose

A

6

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18
Q

number of carbons in sucrose

A

12

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19
Q

why do we make yeast and water a control when comparing different carbohydrates

A

because water does not have any sugar/ carbon in it which yeast needs to grow

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20
Q

what part of a phospholipid is hydrophilic

A

the phosphate group head

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21
Q

what part of a phospholipid is hydrophobic

A

the fatty acid tail

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22
Q

how does a spec work

A

it measures the absorbance

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23
Q

what is an enzyme

A

a catalyst that speeds up chemical reaction without being used in up in the process

24
Q

what is a catalyst

A

they are very specific and they react with a certain substrate to catalyze a narrow class of chemical reactions

25
what is hydrogen peroxide in our enzyme lab
the substrate for our enzyme
26
what is photosynthesis
the process by which plants and certain algae synthesize simple organic molecules from carbon dioxide and water
27
what is replication
the process of copying DNA to make new DNA
28
what is translation
the final step to turn the DNA message into a protein product. The mRNA matches to anticodons on tRNA to bring specific amino acids based on the genetic code
29
what is transcription
this occurs on a regular basis in every single cell if the body and this is the process of using the info stored in DNA to make mRNA
30
why does starch have the most potential energy with yeast
it has repeating glucose units
31
stains combine with what to increase contrast of cells under a microscope
low power, stage all the way up
32
why is staining a step often used when looking at cells under a microscope
increases contrast
33
when you begin to focus the microscope, which combination below is correct
low power, stage all the way up
34
how do we obtain the major macromolecules our bodies need
common foods
35
indicators can test for the presence of
sugars, fats, and proteins
36
in diffusion substances move from a _______ concentration to a ________ concentration
higher, lower
37
Biurets reagent is an indicator for the presence of
proteins
38
Benedicts reagent is an indicator for the presence of
sugar
39
if a cell crenates, it
shrinks
40
why do we say that a 12 carbon sugar has more potential energy than a 6 carbon sugar
more carbons mean more energy and 12 carbons has more potential energy because not all organisms could break down the 12 C to release the energy
41
what is the relationship between variance and standard deviation
take the square root of variance to get SD
42
give the equation to calculate enzyme activity rate
final absorbance reading reading - initial absorbance reading / number of seconds
43
what does an optimal pH give
the highest enzyme activity
44
the function of peroxidase is to turn hydrogen peroxide into
water and oxygen
45
photosynthesis uses carbon dioxide and water to make the final product
glucose
46
why/ how does pH affect enzyme activity
alters structure and lowers activity
47
why do leaf disks float in the solution contains sodium bicarbonate but not in the solution without sodium bicarbonate
bicarbonate is the carbon source - without it no photosynthesis, no oxygen, no floating
48
photosynthesis produces oxygen. What is the process that consumes oxygen and produces ATP for the cell
cellular respiration
49
what is the purpose of soap in the solutions for the leaf disk assay
wets the hydrophobic leaf surface allowing solution to get into the leaf
50
stage of meiosis I in which the homologous pairs line up on the equilateral plate
metaphase I
51
stage of meiosis in which the chromosomes gather into nuclei, and the original cell divides
telophase I
52
stage of meiosis II in which the chromosomes gather into nuclei and the cells divide
telophase II
53
stage of meiosis I in which the chromatin begin to condense following interphase
early prophase I
54
stage of mitosis in which daughter chromosomes reach the poles, the nuclei re-form, and the cell again enters interphase
telophase
55
where in the mouth are taste receptors located
taste buds
56
papillae are visible to the naked eye
true