lab practical 1 Flashcards
a spherical bacterium
coccus
rod-shaped bacteria
bascillus
a spiral-shaped bacterium
spirillum
visible mass of cells
colony
putting bacteria into a medium
innoculation
bacteria used in endospore staining
B.cereus
resting caused by lack of nutrients
endospore
- resistance to heat, radiation, acids & chemicals
- production of calcium dipicolinate
- cell dehydration
- spores can be destroyed in an autoclave
endospores
Differentiate between vegetative cells & endospores
endospore staining
protective protein coat of endospore
exosporium
endospore stain and coutnerstain
-malachite green
-counterstain: safranin
Waxy lipid, makes cells not permeable to most stains.
mycolic acid (acid fast)
primary stain in acid fast is
Carbolfuchsin
- stains acid-fast bacteria pink
Counterstain in acid-fast is
methylene blue
- stains non-acid-fast bacteria blue
decolorizer for acid fast staining
acid alcohol
bacteria used in acid-fast staining
mycobacterium smegmatis and stapholococcus
color of mycobacterium in acid fast stain
pink because they are acid fast
color of staph aureus in acid-fast stain
methylene blue bc they are non-acid fast
establish the difference between acid-fast & non-acid fast cells (differential stain)
acid-fast staining
- E.coli
- P.aeruginosa
- S.aureus
3 bacteria in the pure culture technique
- morphology
- arrangement
- internal structures
Good smears are important to observe
Two types of morphology
bacilli: rods
cocci: spherical
Two types of arrangements
staph: cluster
strep: chain
invented by christian gram to identify between bacteria and eukarya cells
gram staining
gram staining primary stain
crystal violet
gram staining mordant stain
gram’s iodine
gram staining decolorization
ethyl alcohol
gram staining counter stain
safranin
monotrichous flagella
one flagella