exam 1 Flashcards
Given a choice between functionally equivalent designs, the simplest design should be selected.
Ockham’s Razor
- treatable, contagious bacterial disease
- contracted from armadillos
- incubation period = 18 years
- respiratory transmission
Leprosy
yeast fermentation
wine
- first to report cells (cork)
- responsible for the Cell Theory
Robert Hooke
first to observe the life of microorganisms
Antone Van Leeuwenhoek
Father of microbiology
Louis Pasteur
- discovered fermentation
- disproved spontaneous generation
- pasteurization
Louis Pasteur
created germ theory of disease
Robert Koch
Pasteur demonstrated that microbes are responsible for ____ which lead to the connection between microbes and ____
food spoilage / diseases
Pasteur provided the bases for ___ ___ which prevent microbial contamination
aseptic techniques
1) pour meat broth into flask – watch
2) pour meat broth into flask, bend neck — watch
3) pour meat broth into flask, boil – watch
Louis Pasteur’s Experiment steps
what will happen if you fail to bend the neck of the flask in Pasteur’s experiment?
original microbes will boil out and new ones will enter through the top and contaminate culture
When the broth was being heated in the Pasteur experiment, not all organisms were ____
What could have formed in the flask but did not?
killed
endospores formed that are not fueled by boiling / he was lucky that there were no endospores in meat broth
- The same pathogen must be present in every case of the disease.
- The pathogen must be isolated from the diseased host and grown in pure culture.
- The pathogen from the pure culture must cause the disease when it is inoculated into a healthy, susceptible laboratory animal.
- The pathogen must be isolated from the inoculated animal and must be shown to be the original organism.
robert koch’s 4 postulates
according to koch’s postulates, a specific infectious disease is caused by a specific ____
microbe
koch’s postulates help scientists identify the first step in ___ and ___ of the disease
treatment / prevention
Treponema pallidum
HIV
exceptions to Koch’s postulate
- causes syphilis
- cannot be grown in pure culture
Treponema pallidum
- causes AIDS
- can be injected but will not show any signs of disease in the test animals
HIV
first pasteurization of grape juice in 1869
Thomas Bramwell Welch
the origin of mitochondria and chloroplast came from ____
bacteria
DNA passed form our ancestors is described as ____
conserved
Kingdoms of Eukarya
Protista, Fungi, Plantae, Animalia
structure that carries out all functions in a prokaryotic cell
cell membrane
coccus
circle
bacillus
rods
streptococci
chain
staphylococci
clumped together; staph infections
lyme disease and syphilis are caused by ____ bacteria
spiral
no nuclear membrane
usually singular, circular, and haploid
always made of double-stranded DNA
Nucleoid / bacterial chromosomes
usually for storage
inclusion
used for conjugation in certain species
pilus
used for attachment
fimbria
-extracellular material is usually made of polysaccharides
- used for attachment
capsule
-extrachromosomal genetic material
- always made of double-stranded DNA
plasmid
used for motility
flagellum
A jellylike fluid inside the cell in which the organelles are suspended
- “guts” of the cell
cytoplasm
site of protein synthesis
ribosomes
70s ribosomes
Ribosomes in prokaryotic cells
80S ribosomes
larger ribosomes found in eukaryotes
many antibiotics affect ____ ribosomes
70s
- ACTIVE barrier that separates inside of cell from outside
- made of proteins and lipids
- more liquid than solid
cell membrane
A DIFFERENTIAL staining method that distinguishes between two different kinds of bacterial cell walls.
Gram stain
procedure for gram staining
- Crystal violet
- Iodine
- Alcohol
- Safranin
purple stained bacteria that have simple cell walls with a thick layer of peptidoglycan.
Gram-positive bacteria
PINK STAINED bacteria that have complex cell walls with thin layer of peptidoglycan but with lipopolysaccharides.
Gram-negative bacteria
peptidoglycan is only found in ____
bacteria
aka endotoxin
sticks out from the membrane in gram-negative staining
Lipopolysaccharide
interferes with synthesis of peptidoglycan
Penicillin
purpose of an endospore
survival not reproductive
bacillus is ____ bacteria
aerobic
clostridium is ____ bacteria
Anaerobic
what resistance forms can be absolute and still have resistance?
desiccation
low temperatures
Anthrax (Wool Sorter’s Disease)
Bacillus anthracis
causes gas gangrene
Clostridium perfringens
causes tetanus (“lock jaw”)
third deadliest toxin known
Clostridium tetani
causes botulism (food poisoning)
botox
deadliest toxin known
reason why honey is not fed to infants
Clostridium botulinum
does not require an organism to be present
Clostridium botulinum
second deadliest toxin
Diptheria toxin
4 atypical bacteria
Rickettsiae
Chlamydiae
Mycoplasmas
Actinomycetes
obligate intracellular parasites
rocky mountain spotted fever
spread by ticks
Rickettsiae
obligate intracellular parasites
trachoma
number 1 STD
spread by direct human contact
chlamydiae
bacteria that naturally lack a cell wall
smallest known living cells
mycoplasmas
Mold-like in appearance
Streptomyces (produce antibiotics)
Actinomycetes
prokaryotes divide by _____
eukaryotes divide through ____
binary fission
involves mitosis
Bacteria have ___ linkages in cell membrane lipids
Archaea have ___ linkages in cell membrane lipids
ester
ether
3 eukaryotic microorganisms
fungi, protozoa, algae
Molds are often ____cellular
Yeasts are ____cellular
multi / uni
Molds are ___ bacteria
Yeasts are ____ bacteria
aerobic / facultative anaerobic
fungus-like protist that moves like protozoa and is important in the decay process
slime mold
3 types of protozoa
pseudopodia (amoeba)
flagella (euglena)
cilia (paramecium)
importance of protozoa
food chain
diseases
organisms that perform oxygen-evolving photosynthesis and possess chloroplasts
algae
algae can cause a few diseases like ____
Paralytic Shellfish Poisoning (red tide)
HIV is an exception to Koch’s Postulates because
it does not cause AIDS in an experimental host
bacterial structures that have to do with attachment include
Capsules and Fimbriae
after the gram stain procedure, gram positive and negative is obvious after ___
application of safranin
the structure that is required for disease to occur specifically pneumonia or dental cavitites is a
capsule
the shape and arrangement of bacteria associated with the normal bacteria growing on human skin and MRSA is
Staphylococci
bacteria that causes syphillis and lyme disease
spirochetes
barrier function of the cell membrane is because of
lipids
cyanobacteria are not algae because
do not have chlororplasts
Protozoa motility structures include
cilia and pseudopodia
the most abundant life forms on Earth, the Archaea are mostly _____
Anaerobic
movement in most motile prokaryotes is due to
flagella
required by streptococcus pneumonia to cause disease
bacterial capsule
which part of the salmonella cell is responsible for inducing the symptoms of food poisoning?
Lipopolysaccharide
ectoplasm is found in the organism taht causes ___
malaria
all molds can use spores for ____
reproduction
some bacteria use endospores for ____ while all molds use spores for _____
survival / reproduction
molds require the following for growth
water
organic matter
aerobic conditions
penicillin is produced by
a mold
all algae produce ___ __ and some are ____
molecular oxygen / toxic
what bacteria is motile?
spirilla
distinguishing factors of protozoa
ectoplasm and endoplasm
all have pigments
have some members that are motile
have some members that can cause disease in humans
algae
who coined the terms aerobic and anaerobic?
louis pasteur
can cause the immune system to create symptoms that may lead to death
Lipopolysaccharide
the most critical step in gram staining is ___
decolorization
disease common during the civil war and often requires amputation for survival
Clostridium perfringens
- bacteria can cause blindness in humans
Chlamydia
responsible for producing many of the vitamins that we find in health food stores
yeasts
the disease casued by C. botulinum is an _____
intoxication
protozans can be differentiated from other eukaryotes based on their…
cytoplasms
important diseases caused by protozoans are
Malaria and amoebic dysentery