Lab midterm study

Question 1

what are the min and max for the three different micropipettes

Answer 1

p20 (min-2, max-20). p200 (min 20, max-200). p1000 (min-200, max-1000)

Question 2

what is the equation c1v1=c2v2 used for

Answer 2

calculating dilutions.c1= concentration v1= volume c2=

Question 3

what does mise en place mean

Answer 3

grab all the things you need before you start the experiment

Question 4

what is transformation

Answer 4

add plasma DNA to bacteria. can give it two treats

Question 5

what is bla b-lactamase gene

Answer 5

makes protein that breaks down ampicillin antibiotics (constitutive -always making mRNA nd protein )

Question 6

what is a GFP gene

Answer 6

makes protein glow green under UV light (inducible -only makes mRNA and protein when in contact with specific nutrients )

Question 7

what kills e.coli cells

Answer 7

Ampicillin

Question 8

why does diffusion occur

Answer 8

because of collisions between molecules. Diffusion is the net movement of anything from a region of higher concentration to a region of lower concentration.

Question 9

10 ml H2O in H2O -?
10 ml H2Oin 1M sucrose - ?
10 ml in 1M sucrose H2O - ?

Answer 9

10 ml H2O in H2O -Isotonic
10 ml H2Oin 1M sucrose - Hypertonic
10 ml in 1M sucrose H2O - Hypotonic

Question 10

what is a Constitutive expression

Answer 10

means the gene is ALWAYS making mRNA & protein

Question 11

what is Inducible expression

Answer 11

means there is a signal (like a nutrient) to turn expression on
no signal means no mRNA, no protein