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BI543—Pathogenic Microbiology > Lab Midterm > Flashcards

Lab Midterm Flashcards

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1
Q

MSA selective ingredient

A

7.5% NaCl

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2
Q

MSA differential ingredient

A

mannitol

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3
Q

are GP or GN more sensitive to salt?

A

Gram negative

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4
Q

enzyme needed to ferment mannitol?

A

MOH

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5
Q

color of a mannitol fermenter?

A

yellow

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6
Q

key Man+ organism

A

S. aureus

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7
Q

is coagulase performed with serum or plasma?
why?

A

plasma
it contains clotting factors

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8
Q

molecule that forms a clot

A

fibrin

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9
Q

CNS besides S. epidermidis

A

S. haemolyticus

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10
Q

coagulase detects (bound/free) enzyme

A

bound

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11
Q

bile esculin selective ingredient

A

bile

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12
Q

bile esculin differential ingredient

A

esculin

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13
Q

sugar in esculin

A

glucose

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14
Q

what reacts with esculin to give brown-black color?

A

iron

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15
Q

special bile esculin condition for Bacteroides

A

anaerobic environment

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16
Q

organism that would grow on bile esculin

A

E. faecalis

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17
Q

type II cytotoxins cause —- hemolysis

A

beta

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18
Q

y-hemolysis organism

A

E. faecalis

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19
Q

green colored hemolysis

A

alpha

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20
Q

a-hemolysis organism

A

S. pneumoniae

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21
Q

a-hemolysis results from…

A

oxidation of hemoglobin by peroxide to methemoglobin

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22
Q

hemolysin expressing maximal activity under anaerobic conditions

A

streptolysin O (SLO)

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23
Q

optochin inhibits…

A

ATPase

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24
Q

zone required to indicate susceptibility to 5 μg optochin

A

≥ 14mm

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25
Q

organism indicated by optochin sensitivity

A

S. pneumoniae

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26
Q

bacitracin mode of action

A

inhibits cell wall synth

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27
Q

S. pyogenes – bacitracin

A

sensitive

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28
Q

Staphylococcus – bacitracin

A

resistant

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29
Q

Bacillus licheniformis – bacitracin

A

resistant

30
Q

Micrococcus – bacitracin

A

sensitive

31
Q

SXT mode of action

A

inhibits folic acid metabolism

32
Q

Group A strep example

A

S. pyogenes

33
Q

Group B strep example

A

S. agalactiae

34
Q

which strep group grows in the presence of bacitracin?

A

Group B grows
Group A does not grow

35
Q

what is on the Staphaurex beads?

A

antibody against S. aureus

36
Q

DNase positive result

A

colorless halo around DNase positive colonies

37
Q

Example of DNase pos organism

A

S. marscesens

38
Q

CAMP positive

A

arrowhead zone

39
Q

is CAMP factor higher closer or further from the arrowhead?

A

further

40
Q

organism streaked on CAMP plate (not being tested)

A

S. aureus

41
Q

example of pos CAMP organism

A

Streptococcus agalactiae

42
Q

novobiocin mode of action

A

interferes with DNA replication by inhibiting DNA gyrase

43
Q

resistant to novobiocin

A

Staphylococcus saprophyticus

44
Q

steps of growth-dependent microbiology

A

enrichment (selective or differential media)
isolation (pure culture)
identification

45
Q

reducing agent used for

A

anaerobes

46
Q

swaps placed in transport tube with…

A

sterile saline

47
Q

specificity
few false —–

A

ability of test to recognize a pathogen
few false positives

48
Q

sensitivity
few false ——

A

smallest quantity of pathogen that can be detected
few false negatives

49
Q

rare organism, few false positives

A

GNC
ex. N. gonorrhoeae

50
Q

(males/females) give more cells

A

males

51
Q

N. gonorrhoeae swabs are plated on —– agar

A

Thayer-Martin

52
Q

—— used for further confirmation of N. gonorrhoeae

A

oxidase

53
Q

selective media

A

contain poisons or inhibitors which prevent growth of specific competitors

54
Q

differential media

A

indicators that change color
often a pH indicator for acid production

55
Q

sensitivity for finding a cell from a spinal tap smear

A

10^5

56
Q

—— often required for spinal taps

A

culturing

57
Q

—— mL blood needed to inoculate “blood bottles”

A

10-20 mL

58
Q

2 blood bottles

A

aerobic
anaerobic

59
Q

can take 5 days to grow in blood bottles

A

anaerobes

60
Q

can take 2 weeks to grow in blood bottles

A

TB

61
Q

preferred media for IDing UTI organisms

A

MacConkey
EMB

62
Q

—– and —– are very sensitive to acid buildup, so fecal samples must be handled rapidly

A

Salmonella
Shigella

63
Q

—— parasites can be directly observed in fecal samples

A

Giardia

64
Q

first step for bites/wounds

A

selective & differential media

65
Q

if swap doesn’t work on bite/wound

A

syringe aspiration or surgical biopsy

66
Q

chromagar

A

colorimetric substrate broken down to give distinct color

67
Q

cause urethritis (4)

A

Chlamydia
Ureaplasma
Trichomonas
N. gonorrhoeae

68
Q

in (males/females) organisms can be found in exudates by microscopy

A

males

69
Q

media choices for N. gonorroeae

A

chocolate agar
modified TM agar with 4 antibiotics
NYC medium lacking blood, plasma and soluble hemoglobin instead

70
Q

culturing N. gonorrhoeae requires what environment?

A

3-7% CO2 incubator

71
Q

N. gonorrhoeae
oxidase?
gram?
arrangement/shape?

A

oxidase pos
G N
diplococci

BI543—Pathogenic Microbiology (10 decks)

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