Lab Midterm Flashcards
volume to be taken up by micropipette is found in _________
volumeter
1 or 2nd stop for retrieving fluid
first
little slidey thing with markings under stage of microscope
iris diaphragm knob
circley thing beneath stage with the two screws in it
condenser
limiting factor when moving to higher magnification - reason we need oil
resolution
resolution
ability to distinguish small things close together
resolving power is a function of…
quality of lenses
wavelength of light
index of refraction
parfocal
if one objective is in focus, others will be very close to being in focus
oil is used because…
it has the same index of refraction as glass, so the least amount of light is blurred
lens used with oil
black stripe, 100x
steps to Kohler illumination
- reduce diaphragm
- focus condenser
- center illumination area
- open up diaphragm
steps to clean microscope
- clean all oil off slides
- turn dimmer switch to 1; power off
- secure cord under stage
- clean 100x with lens paper and spray
- clean stage, condenser, everything else
4x conversion factor
1 mark = 25 μm
10x conversion factor
1 mark = 10 μm
40x conversion factor
1 mark = 2.5 μm
100x conversion factor
1 mark = 1 μm
prevalence of neutrophils
80% of WBCs
prevalence of lymphocytes
25% of WBCs
prevalence of monocytes
2-10% of WBCs
prevalence of eosinophils
1-3% of WBCs
prevalence of basophils
1% of WBCs
very corner square of hemocytometer area
volume fluid
1 mm^2
0.1 μL
DF =
1 / dilution
to go from titer in dilution to titer in original sample…
multiply cells/μL by DF
why do O people have anti A/B antibodies?
antibodies develop upon exposure to commensal organisms with antigens similar to A & B
cross match test involves…
donor RBC with recipient serum
3 categories of plasma proteins
albumins
globulins
fibrinogen
serum vs plasma
serum = fluid found in plasma - does not include coagulants or blood cells - remains after blood has clotted
plasma = includes clotting factors - present when blood has been prevented from clotting
blood temp
38° C
