Lab Midterm

Question 1

volume to be taken up by micropipette is found in _________

Answer 1

volumeter

Question 2

1 or 2nd stop for retrieving fluid

Answer 2

first

Question 3

little slidey thing with markings under stage of microscope

Answer 3

iris diaphragm knob

Question 4

circley thing beneath stage with the two screws in it

Answer 4

condenser

Question 5

limiting factor when moving to higher magnification - reason we need oil

Answer 5

resolution

Question 6

resolution

Answer 6

ability to distinguish small things close together

Question 7

resolving power is a function of…

Answer 7

quality of lenses

wavelength of light

index of refraction

Question 8

parfocal

Answer 8

if one objective is in focus, others will be very close to being in focus

Question 9

oil is used because…

Answer 9

it has the same index of refraction as glass, so the least amount of light is blurred

Question 10

lens used with oil

Answer 10

black stripe, 100x

Question 11

steps to Kohler illumination

Answer 11

1. reduce diaphragm
2. focus condenser
3. center illumination area
4. open up diaphragm

Question 12

steps to clean microscope

Answer 12

1. clean all oil off slides
2. turn dimmer switch to 1; power off
3. secure cord under stage
4. clean 100x with lens paper and spray
5. clean stage, condenser, everything else

Question 13

4x conversion factor

Answer 13

1 mark = 25 μm

Question 14

10x conversion factor

Answer 14

1 mark = 10 μm

Question 15

40x conversion factor

Answer 15

1 mark = 2.5 μm

Question 16

100x conversion factor

Answer 16

1 mark = 1 μm

Question 17

prevalence of neutrophils

Answer 17

80% of WBCs

Question 18

prevalence of lymphocytes

Answer 18

25% of WBCs

Question 19

prevalence of monocytes

Answer 19

2-10% of WBCs

Question 20

prevalence of eosinophils

Answer 20

1-3% of WBCs

Question 21

prevalence of basophils

Answer 21

1% of WBCs

Question 22

very corner square of hemocytometer area

volume fluid

Answer 22

1 mm^2

0.1 μL

Question 23

DF =

Answer 23

1 / dilution

Question 24

to go from titer in dilution to titer in original sample…

Answer 24

multiply cells/μL by DF

Question 25

why do O people have anti A/B antibodies?

Answer 25

antibodies develop upon exposure to commensal organisms with antigens similar to A & B

Question 26

cross match test involves…

Answer 26

donor RBC with recipient serum

Question 27

3 categories of plasma proteins

Answer 27

albumins

globulins

fibrinogen

Question 28

serum vs plasma

Answer 28

serum = fluid found in plasma - does not include coagulants or blood cells - remains after blood has clotted

plasma = includes clotting factors - present when blood has been prevented from clotting

Question 29

blood temp

Answer 29

38° C