[LAB] Lab Safety and Microscopy Flashcards
How microorganisms are transmitted
chain of infection
- Instituted by CDC in 1987
- All patients are considered to be possible carriers of bloodborne pathogens
Universal Precaution
- Not limited to bloodborne pathogens
- Consider ALL body fluids and most body substances to be potentially infectious
- Do not recommend handwashing after removing gloves unless visual contamination is present
Body Substance Isolation
Who made the standard precautions?
Center for Disease and Control (CDC)
Healthcare Infection Control Practices Advisory Committee (HICPAC)
- combined UP and BSI guidelines in 1996 by CDC and the Healthcare Infection Control Practices Advisory Committee (HICPAC)
Standard Precautions
A law monitored and enforced by OSHA that indicates: any accidental exposure to a possible bloodborne pathogen must be immediately reported to a supervisor.
Occupational Exposure to Bloodborne Pathogens Standard
What is the primary mode of transmission?
hand contact
dilution of sodium hypochlorite for disinfecting the sink
1:5 or 1:10
When chemical spills came in contact with your eye and skin, how long will you wash your eye or skin with water?
15 minutes
True or False.
Acid should always be added to water
True
Who made the symbol for 4 hazards (health, fire, specific, and reactivity)?
National Fire Protection Association
A document that provides workers with procedures for safety handling or working with a particular substance.
Material Safety Data Sheet
Enumerate the meaning of RACE.
Rescue
Alarm
Contain
Extinguish
An instrument that magnifies objects that are too small to be seen by the naked eye, producing an image in which the objects appear larger.
Microscope
Identify the part of the microscope.
- carries the optical parts in the upper part of the microscope
head
Identify the part of the microscope.
- Acts as support
- Also carries the microscopic illuminators
base
Identify the part of the microscope.
- Connects the base and the head of the microscope
- Support to the head of the microscope
arm
Identify the part of the microscope.
- Ocular
- Part used to look through the microscope
- Found at the top of the microscope
- Standard magnification is 10x
eyepiece
What is the standard magnification?
10x
Identify the part of the microscope.
- Major lenses used for specimen visualization
- Has a magnification power of 4x,10x, 40x, and 100x (scanner, LPO, HPO, and OIO ̧ respectively)
objective lenses
Identify the part of the microscope.
- Revolving turret
- Holds the objective lenses
nosepiece
Identify the part of the microscope.
- Hole in the microscopic stage
- The light is transmitted from the source to the stage
aperture
Identify the part of the microscope.
- Light source
- Used instead of a mirror
microscopic illuminator
Identify the part of the microscope.
- Used to collect and focus light
- Ensures clear, and sharp images with high magnification
condenser
Identify the part of the microscope.
- controls the amount of light that reaches the specimen
diaphragm
Identify the type of microscope.
- Frequently used in the clinical laboratory
- Objects appear dark on a light background
- Used in routine urinalysis
bright-field
Identify the type of microscope.
- Enhances visualization of elements with low refractive indices
- Used for gaining contrast in a translucent specimen without staining
phase-contrast
Identify the type of microscope.
- Use of polarized light aids in the identification of crystals, oval fat bodies, fatty casts, and crystals
- Elements seen under polarized light are birefringent
polarizing
Identify the type of microscope.
- Aids in the identification of Treponema pallidum
- To enhance visualization of specimens that cannot be seen easily viewed with a bright-field microscope
- Often used for unstained specimens
- Specimen appears light against the black background
dark-field
Identify the type of microscope.
- Used to detect bacteria and viruses within cells and tissues
- Allows visualization of naturally fluorescent microorganisms or those stained by a fluorescence dye including labeled antigens and antibodies
fluorescence
Identify the type of microscope.
- Produces a three-dimensional microscopy image and layer-by-layer imaging of a specimen
interference-contrast
