[LAB] Lab Safety and Microscopy Flashcards

1
Q

How microorganisms are transmitted

A

chain of infection

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2
Q
  • Instituted by CDC in 1987
  • All patients are considered to be possible carriers of bloodborne pathogens
A

Universal Precaution

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3
Q
  • Not limited to bloodborne pathogens
  • Consider ALL body fluids and most body substances to be potentially infectious
  • Do not recommend handwashing after removing gloves unless visual contamination is present
A

Body Substance Isolation

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4
Q

Who made the standard precautions?

A

Center for Disease and Control (CDC)
Healthcare Infection Control Practices Advisory Committee (HICPAC)

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5
Q
  • combined UP and BSI guidelines in 1996 by CDC and the Healthcare Infection Control Practices Advisory Committee (HICPAC)
A

Standard Precautions

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6
Q

A law monitored and enforced by OSHA that indicates: any accidental exposure to a possible bloodborne pathogen must be immediately reported to a supervisor.

A

Occupational Exposure to Bloodborne Pathogens Standard

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7
Q

What is the primary mode of transmission?

A

hand contact

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8
Q

dilution of sodium hypochlorite for disinfecting the sink

A

1:5 or 1:10

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9
Q

When chemical spills came in contact with your eye and skin, how long will you wash your eye or skin with water?

A

15 minutes

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10
Q

True or False.

Acid should always be added to water

A

True

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11
Q

Who made the symbol for 4 hazards (health, fire, specific, and reactivity)?

A

National Fire Protection Association

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12
Q

A document that provides workers with procedures for safety handling or working with a particular substance.

A

Material Safety Data Sheet

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13
Q

Enumerate the meaning of RACE.

A

Rescue
Alarm
Contain
Extinguish

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14
Q

An instrument that magnifies objects that are too small to be seen by the naked eye, producing an image in which the objects appear larger.

A

Microscope

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15
Q

Identify the part of the microscope.

  • carries the optical parts in the upper part of the microscope
A

head

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16
Q

Identify the part of the microscope.

  • Acts as support
  • Also carries the microscopic illuminators
A

base

17
Q

Identify the part of the microscope.

  • Connects the base and the head of the microscope
  • Support to the head of the microscope
A

arm

18
Q

Identify the part of the microscope.

  • Ocular
  • Part used to look through the microscope
  • Found at the top of the microscope
  • Standard magnification is 10x
A

eyepiece

19
Q

What is the standard magnification?

A

10x

20
Q

Identify the part of the microscope.

  • Major lenses used for specimen visualization
  • Has a magnification power of 4x,10x, 40x, and 100x (scanner, LPO, HPO, and OIO ̧ respectively)
A

objective lenses

21
Q

Identify the part of the microscope.

  • Revolving turret
  • Holds the objective lenses
A

nosepiece

22
Q

Identify the part of the microscope.

  • Hole in the microscopic stage
  • The light is transmitted from the source to the stage
A

aperture

23
Q

Identify the part of the microscope.

  • Light source
  • Used instead of a mirror
A

microscopic illuminator

24
Q

Identify the part of the microscope.

  • Used to collect and focus light
  • Ensures clear, and sharp images with high magnification
A

condenser

25
Q

Identify the part of the microscope.

  • controls the amount of light that reaches the specimen
A

diaphragm

26
Q

Identify the type of microscope.

  • Frequently used in the clinical laboratory
  • Objects appear dark on a light background
  • Used in routine urinalysis
A

bright-field

27
Q

Identify the type of microscope.

  • Enhances visualization of elements with low refractive indices
  • Used for gaining contrast in a translucent specimen without staining
A

phase-contrast

28
Q

Identify the type of microscope.

  • Use of polarized light aids in the identification of crystals, oval fat bodies, fatty casts, and crystals
  • Elements seen under polarized light are birefringent
A

polarizing

29
Q

Identify the type of microscope.

  • Aids in the identification of Treponema pallidum
  • To enhance visualization of specimens that cannot be seen easily viewed with a bright-field microscope
  • Often used for unstained specimens
  • Specimen appears light against the black background
A

dark-field

30
Q

Identify the type of microscope.

  • Used to detect bacteria and viruses within cells and tissues
  • Allows visualization of naturally fluorescent microorganisms or those stained by a fluorescence dye including labeled antigens and antibodies
A

fluorescence

31
Q

Identify the type of microscope.

  • Produces a three-dimensional microscopy image and layer-by-layer imaging of a specimen
A

interference-contrast