[LAB] Chemical Examination of Urine

Question 1

What will happen if you leave excess urine on the reagent strip after removing it from the specimen?

Answer 1

It will cause runover between reagent pads

Question 2

What readings are affected when you failed to mix a specimen before inserting the reagent strip?

Answer 2

blood reading and leukocyte reading

Question 3

What will be affected if you test a refrigerated specimen that has not warmed to room temperature?

Answer 3

enzymatic reactions

Question 4

What reagent strip reaction requires the longest reaction time?

Answer 4

leukocyte esterase

Question 5

Quality control of reagent strips is performed:

a) Using positive and negative controls
b) When results are questionable
c) Per laboratory policy
d) All of the above

Answer 5

(D) all of the above

Question 6

All of the following are important to protect the integrity of reagent strips except:

a) Removing the desiccant from the bottle
b) Storing in an opaque bottle
c) Storing at room temperature
d) Resealing the bottle after removing a strip

Answer 6

a) Removing the desiccant from the bottle

Question 7

What is the principle of the reagent strip test for pH?

Answer 7

double indicator reaction

Question 8

A urine specimen with a pH of 9.0 should be ____.

Answer 8

recollected

Question 9

The primary chemical on the reagent strip in the Micral-Test for microalbumin binds to:

Answer 9

Antihuman albumin antibody

Question 10

The principle of the protein-high pad on the Multistix Pro reagent strip is the:

Answer 10

Protein error of indicators

Question 11

The principle of the protein-low reagent pad on the Multistix Pro is the:

a) Binding of albumin to sulphonphthalein dye
b) Immunological binding of albumin to antibody
c) Reverse protein error of indicators reaction
d) Enzymatic reaction between albumin and dye

Answer 11

a) Binding of albumin to sulphonphthalein dye

Question 12

The principle of the creatinine reagent pad on microalbumin reagent strips is the:

Answer 12

Pseudoperoxidase reaction

Question 13

What is the purpose of performing the albumin:creatinine ratio?

Answer 13

Estimate the glomerular filtration rate

Question 14

The principle of the reagent strip tests for glucose is the:

Answer 14

Double sequential enzyme reaction

Question 15

The primary reason for performing a Clinitest is to:
a) Check for high ascorbic acid levels
b) Confirm a positive reagent strip glucose
c) Check for newborn galactosuria
d) Confirm a negative glucose reading

Answer 15

c) Check for newborn galactosuria

Question 16

What are the 3 intermediate products of fat metabolism?

Answer 16

• Acetoacetic acid
• B-hydroxybutyric acid
• Acetone

Question 17

The most significant reagent strip test that is associated with a positive ketone result:

Answer 17

glucose

Question 18

The primary reagent in the reagent strip test for ketones is:

Answer 18

sodium nitroprusside

Question 19

The principle of the reagent strip test for blood is based on the:
a) Binding of heme and a chromogenic dye
b) Peroxidase activity of heme
c) Reaction of peroxide and chromogen
d) Diazo activity of heme

Answer 19

b) Peroxidase activity of heme

Question 20

A speckled pattern on the blood pad of the reagent strip indicates:

Answer 20

hematuria

Question 21

The principle of the reagent strip test for bilirubin is the:

Answer 21

diazo reagent

Question 22

The reagent in the Multistix reaction for urobilinogen is:

Answer 22

p-Dimethylaminobenzaldehyde

Question 23

The reagent strip test for nitrite uses what reaction?

Answer 23

Greiss reaction

Question 24

What is the principle of the leukocyte esterase reagent strip test?

Answer 24

diazo reaction

Question 25

The principle of the reagent strip test for specific gravity uses the dissociation constant of a(n):

Answer 25

polyelectrolyte

Question 26

A specific gravity of 1.005 would produce the reagent strip color:

Answer 26

blue

Question 27

Specific gravity readings on a reagent strip are affected by:

Answer 27

alkaline urine

Question 28

What is the greatest source of false-negative glucose?

Answer 28

technical error

Question 29

A cold precipitation test that reacts equally in all forms of proteins.

Answer 29

Sulfosalicylic Acid (SSA) Precipitation Test

Question 30

Important in the detection and monitoring of diabetes mellitus.

Answer 30

glucose