[LAB] Chemical Examination of Urine Flashcards
What will happen if you leave excess urine on the reagent strip after removing it from the specimen?
It will cause runover between reagent pads
What readings are affected when you failed to mix a specimen before inserting the reagent strip?
blood reading and leukocyte reading
What will be affected if you test a refrigerated specimen that has not warmed to room temperature?
enzymatic reactions
What reagent strip reaction requires the longest reaction time?
leukocyte esterase
Quality control of reagent strips is performed:
a) Using positive and negative controls
b) When results are questionable
c) Per laboratory policy
d) All of the above
(D) all of the above
All of the following are important to protect the integrity of reagent strips except:
a) Removing the desiccant from the bottle
b) Storing in an opaque bottle
c) Storing at room temperature
d) Resealing the bottle after removing a strip
a) Removing the desiccant from the bottle
What is the principle of the reagent strip test for pH?
double indicator reaction
A urine specimen with a pH of 9.0 should be ____.
recollected
The primary chemical on the reagent strip in the Micral-Test for microalbumin binds to:
Antihuman albumin antibody
The principle of the protein-high pad on the Multistix Pro reagent strip is the:
Protein error of indicators
The principle of the protein-low reagent pad on the Multistix Pro is the:
a) Binding of albumin to sulphonphthalein dye
b) Immunological binding of albumin to antibody
c) Reverse protein error of indicators reaction
d) Enzymatic reaction between albumin and dye
a) Binding of albumin to sulphonphthalein dye
The principle of the creatinine reagent pad on microalbumin reagent strips is the:
Pseudoperoxidase reaction
What is the purpose of performing the albumin:creatinine ratio?
Estimate the glomerular filtration rate
The principle of the reagent strip tests for glucose is the:
Double sequential enzyme reaction
The primary reason for performing a Clinitest is to:
a) Check for high ascorbic acid levels
b) Confirm a positive reagent strip glucose
c) Check for newborn galactosuria
d) Confirm a negative glucose reading
c) Check for newborn galactosuria
What are the 3 intermediate products of fat metabolism?
- Acetoacetic acid
- B-hydroxybutyric acid
- Acetone
The most significant reagent strip test that is associated with a positive ketone result:
glucose
The primary reagent in the reagent strip test for ketones is:
sodium nitroprusside
The principle of the reagent strip test for blood is based on the:
a) Binding of heme and a chromogenic dye
b) Peroxidase activity of heme
c) Reaction of peroxide and chromogen
d) Diazo activity of heme
b) Peroxidase activity of heme
A speckled pattern on the blood pad of the reagent strip indicates:
hematuria
The principle of the reagent strip test for bilirubin is the:
diazo reagent
The reagent in the Multistix reaction for urobilinogen is:
p-Dimethylaminobenzaldehyde
The reagent strip test for nitrite uses what reaction?
Greiss reaction
What is the principle of the leukocyte esterase reagent strip test?
diazo reaction
The principle of the reagent strip test for specific gravity uses the dissociation constant of a(n):
polyelectrolyte
A specific gravity of 1.005 would produce the reagent strip color:
blue
Specific gravity readings on a reagent strip are affected by:
alkaline urine
What is the greatest source of false-negative glucose?
technical error
A cold precipitation test that reacts equally in all forms of proteins.
Sulfosalicylic Acid (SSA) Precipitation Test
Important in the detection and monitoring of diabetes mellitus.
glucose
