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AUBF > Cerebrospinal Fluid > Flashcards

Cerebrospinal Fluid Flashcards

1
Q

functions of CSF

A
  1. Supply nutrients
  2. Remove wastes
  3. Maintain intracranial pressure
  4. Cushion (BBB)
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2
Q

Identify the layer of meninges:

hard mother

lines the skull and vertebral canal

A

dura mater

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3
Q

Identify the layer of meninges:

filamentous inner membrane

spiderweb-like

A

arachnoid

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4
Q

Identify the layer of meninges:

gentle mother

thin membrane lining the surfaces of brain and spinal cord

A

pia mater

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5
Q

Where is the CSF produced?

A

choroid plexuses

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6
Q

How much is the production of CSF per hour?

A

20mL/hr

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7
Q

CSF RI in adults

A

90-150mL

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8
Q

CSF RI in neonates

A

10-60mL

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9
Q

tigh-fitting junctures that prevent the passage of antibodies, leukocytes, protein, and some medications in the choroid plexuses

A

Blood Brain Barrier

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10
Q

specimen collection of CSF

A

lumbar puncture

(between 3rd and 4th / 4th and 5th lumbar vertebra)

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11
Q

Identify the tube number in CSF collection:

chemical and serological tests

A

Tube 1

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12
Q

Identify the tube number in CSF collection:

microbiology

A

Tube 2

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13
Q

Identify the tube number in CSF collection:

hematology (cell count)

A

Tube 3

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14
Q

What is the purpose of fourth tube CSF collection?

A

confirmatory test for microbiology lab

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15
Q

If tube 1 (chem + sero) cannot be tested immediately, what is its required temperature for storage?

A

frozen

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16
Q

If tube 2 (microbio) cannot be tested immediately, what is its required temperature for storage?

A

room temperature

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17
Q

If tube 3 (hema) cannot be tested immediately, what is its required temperature for storage?

A

refrigerated up to 4hrs

18
Q

What is the term used to describe CSF supernatant that is pink, orange, or yellow?

RBC degradation products, ↑ bilirubin and protein, carotene and melanoma

A

xantochromia

19
Q

What is the significance of having protein in the CSF?

A

Disorders affecting BBB

20
Q

3 Indications of traumatic tap collection

A
  1. uneven blood distribution
  2. clot formation
  3. xanthrochromic supernatant
21
Q

What is the cell count that is routinely performed on CSF specimens?

A

WBC Count

22
Q

lysing factor used in WBC count to lyse RBCs

A

3% glacial acetic acid

23
Q

What is the stain used during WBC count to stain WBCs?

A

Methylene Blue

24
Q

Number of cells counted for differential count

A

100 cells

25
Q

What is the stain used for differential counting?

A

Wright’s stain

26
Q

What is the purpose of adding albumin during cytocentrifugation?

A

Increases cell yield and decreases cell distortion

27
Q

2 cells normally found in CSF specimen

A

monocytes and lymphocytes

28
Q

increased lymphocytes and monocytes

A

pleocytosis

29
Q

indication of HIV infection and AIDs

A

lymphocytes

30
Q

indication of coccidioidal meningitis

A

eosinophils

30
Q

indication of Guillain-Baree syndrome and Sarcoidosis

A

Plasma cells

31
Q

What is the chemical test most frequently performed on CSF?

A

Protein determination

32
Q

RI for total CSF protein

A

15-45mg/dL

33
Q

What makes up most of CSF protein?

A

albumin

34
Q

What composes alpha globulins?

A

haptoglobin and ceruloplasmin

35
Q

Major beta globulin present in CSF

A

Transferrin

36
Q

Identify the type of meningitis:

neutrophils present

A

bacterial meningitis

37
Q

Identify the type of meningitis:

  • normal glucose
  • normal lactate
A

viral meningitis

38
Q

Identify the type of meningitis:

  • presence of weblike clot or pellicle
  • (+) M. tuberculosis
A

mycobacterial (tubercular) meningitis

39
Q

Identify the type of meningitis:

(+) India ink test

Cryptococcus neoformans

A

fungal meningitis

AUBF (9 decks)

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