Lab Exam 2 Flashcards
Evolution of Viruses: 3 hypthesises
- Regressive
- Progressive or Escapist
- Self-Replicating
Regressive hypothesis
viruses evolved from free-living cells or from intracellular prokaryotic
Self-replicating hypothesis
viruses may have originated form self-replicating entities similar to transposons or other mobile genetic elements
Progressive or Escapist hypothesis
Viruses originated from pieces of RNA and DNA that escaped from a host cell and gained ability to move between cells
Virus shapes:Filamentous
long, thin worm-like shape
Virus shapes: Isometric
spherical-shape
Virus shapes: Enveloped
have membranes that surround capsids
Virus shapes: Complex/head and tail
infect bacteria and have a head that is similar to icon a hedral viruses and a tail shaped like helical viruses
Dna viruses
double stranded; replication takes place in nucleus; few have DNA polymerase and can replicate in host cell’s cytoplasm
Rna viruses
usually single-stranded; replication takes place in cytoplasm; mutation happens at a very high rate because RNA polymerase does not proofread
Four steps of viral infection
- Attachment
- Entry
- Replication and assembly
- Egress (release)
Attachment
receptors on cell bind to virus capsid protein or envelope glycoproteins
Entry
virus enters eukaryotic cells by endocytosis or if envelope by fusions w cell membrane
Replication and assembly
depends on RNA, DNA and retrovirius and reverse transcriptase
Egress
release; may involve lysis and death of host cells, may involved budding (not kill cell)
Lytic cycle
lyse the host cell after replication
lysogenic cycle
do not immediately lyse host cell; bacterial phage genome integrates with host genome and replicates with it without destroying cell; when conditions deteriorate the virus lyses the cell
Reverse transcriptase virus
never occurs in an uninfected host cell - the enzyme reverse transcriptase is only derived from the expression of viral genes within the infected host cell
vaccines
primary method of controlling viral disease; prepared using live, killed or molecular sub units of viruses
prion
proteinaceous infectious particles; no DNA or RNA; cause fatal neurodegenerative diseases, not destroyed by cooking
Viriods
small circles of RNA Iplants)
prokaryote structure
no membrane-bound organelles; no nucleus; DNA in nucleoid; free ribsomes
Extremophiles
adapted to grow under extreme conditions (deeps sea, heat, dry, cold, radiation)
Acidophiles: extreme
low pH
Alkaliphiles: extreme
high pH
thermophiles: extreme
high temps
hyperthermophiles
highest temps
psychrophiles
15 celecius or lower
Halophiles
high salt
osmsophiles
high sugar
cyanobacteria
photosynthesis; some are Nitrogen fixers; ‘blooms’ can make toxins
Plasmids
extra DNA rings with few genes; replicate independently; add diversity
photoautotrops
use energy from sun and carbon from carbon dioxide
chemoheterotrophs
obtain both energy and carbon from an organic chemical source